40 种水稻 E2 泛素连接酶与 17 种水稻 ARM-U-box E3 泛素连接酶的分类和相互作用模式。
Classification and interaction modes of 40 rice E2 ubiquitin-conjugating enzymes with 17 rice ARM-U-box E3 ubiquitin ligases.
机构信息
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
出版信息
Biochem Biophys Res Commun. 2014 Feb 21;444(4):575-80. doi: 10.1016/j.bbrc.2014.01.098. Epub 2014 Jan 29.
Rice, a monocot model crop, contains at least 48 putative E2 ubiquitin (Ub)-conjugating enzymes. Based on homology comparisons with 40 Arabidopsis E2 proteins and 35 human E2s, 48 rice E2s were classified into 15 different groups. Yeast two-hybrid analyses using the U-box-domain regions of armadillo (ARM)-U-box E3 Ub-ligases and the Ub-conjugating (UBC) domains of E2s showed that, among 40 rice E2s, 11 E2s accounted for 70% of the interactions with 17 ARM-U-box E3s. Thus, a single E2 could interact with multiple ARM-U-box E3s, suggesting the presence of E2 hubs for E2-E3 interactions in rice. Rice SPL11 ARM-U-box E3 displayed distinct self-ubiquitination patterns, including poly-ubiquitination, mono-ubiquitination, or no ubiquitination, depending on different E2 partners. This suggests that the mode of ubiquitination of SPL11 E3 is critically influenced by individual E2s.
水稻是单子叶模式作物，至少含有 48 种假定的 E2 泛素（Ub）连接酶。基于与 40 种拟南芥 E2 蛋白和 35 种人类 E2s 的同源性比较，将 48 种水稻 E2s 分为 15 个不同的组。使用臂板蛋白（ARM）-U 盒 E3 Ub 连接酶的 U 盒结构域和 E2s 的 Ub 连接（UBC）结构域进行酵母双杂交分析表明，在 40 种水稻 E2s 中，11 种 E2s 与 17 种 ARM-U 盒 E3s 的相互作用占 70%。因此，单个 E2 可以与多个 ARM-U 盒 E3 相互作用，这表明水稻中存在 E2-E3 相互作用的 E2 集线器。水稻 SPL11 ARM-U 盒 E3 显示出不同的自身泛素化模式，包括多聚泛素化、单泛素化或无泛素化，这取决于不同的 E2 伴侣。这表明 SPL11 E3 的泛素化模式受到单个 E2s 的显著影响。
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