Pandey Usha, Kameswaran Mythili, Dev Sarma Haladhar, Samuel Grace
Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Appl Radiat Isot. 2014 Apr;86:52-6. doi: 10.1016/j.apradiso.2013.12.036. Epub 2014 Jan 8.
The anti CD20 antibody Rituximab was conjugated with para isothiocyanato benzyl diethylene triamine penta acetic acid (p-NCS-Bz-DTPA) and subsequent radiolabeling with (99m)Tc was carried out via the (99m)Tc carbonyl synthon. The (99m)Tc labeled antibody conjugate exhibited >95% radiochemical purity after purification and retained good in vitro stability when studied up to 24h at room temperature. In vitro cell binding studies carried out in Raji cells expressing CD20 antigen validated the biological efficacy of the preparation.
抗CD20抗体利妥昔单抗与对异硫氰酸苄基二乙烯三胺五乙酸（p-NCS-Bz-DTPA）偶联，随后通过99m锝羰基合成子进行99mTc放射性标记。纯化后，99mTc标记的抗体偶联物显示出>95%的放射化学纯度，并且在室温下进行长达24小时的研究时保持良好的体外稳定性。在表达CD20抗原的Raji细胞中进行的体外细胞结合研究验证了该制剂的生物学功效。
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