USDA, ARS Western Human Nutrition Research Center, 430 W. Health Sciences Drive, Davis, CA 95616, USA; Department of Nutrition, University of California, One Shields Ave., Davis, CA 95616, USA.
USDA, ARS Western Human Nutrition Research Center, 430 W. Health Sciences Drive, Davis, CA 95616, USA.
Food Chem. 2014 Jun 15;153:60-5. doi: 10.1016/j.foodchem.2013.12.033. Epub 2013 Dec 14.
Recent discoveries of matrix interferences by haptocorrin (HC) in human milk and serum show that past analyses of vitamin B12 in samples with high HC content might have been inaccurate (Lildballe et al., 2009; Carmel & Agrawal, 2012). We evaluated two competitive enzyme-binding immunoassays for serum/plasma (IMMULITE and SimulTRAC-SNB) for B12 analysis in human milk. B12-recovery rates (United States Environmental Protection Agency, 2007) were determined to be 78.9 ± 9.1% with IMMULITE and 225 ± 108% (range 116-553%) using SimulTRAC-SNB, most likely due to the presence of excess HC. HC-interferences were not observed with the IMMULITE assay, rendering previously reported mandatory HC-removal (Lildballe et al., 2009) unnecessary. Linearity continued at low B12-concentrations (24-193 pM; r(2)>0.985). Milk B12 concentrations from Bangladeshi women (72-959 pM) were significantly lower than those from California (154-933 pM; p<0.0001) showing IMMULITE's robustness against the complex milk matrix and its ability to measure low milk B12 concentrations.
最近在人乳和血清中发现了基质干扰物 haptocorrin (HC)，这表明过去对高 HC 含量样本中维生素 B12 的分析可能不准确（Lildballe 等人，2009 年；Carmel 和 Agrawal，2012 年）。我们评估了两种用于人乳中 B12 分析的竞争性酶结合免疫测定法（IMMULITE 和 SimulTRAC-SNB）。B12 回收率（美国环境保护署，2007 年）分别为 78.9±9.1%（使用 IMMULITE）和 225±108%（范围 116-553%）（使用 SimulTRAC-SNB），这很可能是由于存在过量的 HC。IMMULITE 测定法未观察到 HC 干扰，从而使之前报告的强制性 HC 去除（Lildballe 等人，2009 年）变得不必要。线性关系在低 B12 浓度下（24-193 pM；r(2)>0.985）持续存在。孟加拉国妇女的奶 B12 浓度（72-959 pM）明显低于加利福尼亚州妇女的奶 B12 浓度（154-933 pM；p<0.0001），这表明 IMMULITE 具有对复杂奶基质的稳健性，以及测量低奶 B12 浓度的能力。
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