卵泡年龄对肉牛妊娠率的影响。
The effect of follicle age on pregnancy rate in beef cows.
作者信息
Abreu F M, Geary T W, Cruppe L H, Madsen C A, Jinks E M, Pohler K G, Vasconcelos J L M, Day M L
机构信息
Department of Animal Sciences, The Ohio State University, Columbus 43210.
出版信息
J Anim Sci. 2014 Mar;92(3):1015-21. doi: 10.2527/jas.2013-6760. Epub 2014 Feb 3.
The effect of the age of the ovulatory follicle on fertility in beef cows was investigated. Multiparous (n = 171) and primiparous (n = 129) postpartum beef cows in 2 groups (G1 and G2) received estradiol benzoate (EB; 1 mg/500 kg BW, intramuscular [i.m.]) 5.5 d (G1; n = 162) and 6.5 d (G2; n = 138) after the final GnRH of a synchronization program (5d CO-Synch + CIDR) to induce emergence of a new follicular wave (NFW), followed by prostaglandin F2α (PGF(2α); 25 mg, i.m.) administration either 5.5 d ("young" follicle, YF; n = 155) or 9.5 d ("mature" follicle, MF; n = 145) after EB. Estrous detection coupled with AI 12 h later (estrus-AI) was performed for 60 h (MF) and 84 h (YF) after PGF(2α); cows not detected in estrus within this period received timed AI (TAI) coupled with GnRH at 72 and 96 h, respectively. Within the first 72 h after PGF(2α), more (P < 0.01) cows in the MF (76.3%) than YF treatment (47.7%) exhibited estrus, but through 96 h, the proportion detected in estrus (P < 0.05) and interval from PGF(2α) to estrus (P < 0.01) were greater in the YF than MF treatment (88.6% vs. 76.3%, 78.9 ± 0.8 vs. 57.5 ± 1.6 h, respectively). Age of the ovulatory follicle at AI was greater (P < 0.01) in the MF (9.32 ± 0.04 d) than YF (6.26 ± 0.02 d) treatment, but follicle diameter at AI and pregnancy rates did not differ between MF (13.1 ± 0.2 mm; 72.0%) and YF (12.9 ± 0.1 mm; 67.1%) treatments. Regardless of treatment, the diameter of the ovulatory follicle at AI and pregnancy rate were greater (P < 0.01) with estrus-AI (13.1 ± 0.1 mm; 75.0%) than TAI (12.6 ± 0.2 mm; 55.4%). Cows in the MF treatment that initiated a second NFW after EB but before PGF(2α) (MF2; n = 47) were induced to ovulate with GnRH and TAI at 72h, when ovulatory follicles were 4 d old and 10.2 ± 0.2 mm in diameter. Pregnancy rate for TAI (51.1%) in MF2 did not differ from TAI pregnancy rate (55.4%) across the MF and YF treatments. In summary, the age of the ovulatory follicle affected interval to estrus and AI but did not influence pregnancy rate in suckled beef cows.
研究了排卵卵泡年龄对肉牛繁殖力的影响。将两组（G1和G2）经产（n = 171）和初产（n = 129）的产后肉牛，在同步程序（5天CO-Synch + CIDR）的最后一次促性腺激素释放激素（GnRH）后5.5天（G1；n = 162）和6.5天（G2；n = 138），注射苯甲酸雌二醇（EB；1 mg/500 kg体重，肌肉注射[i.m.]）以诱导新卵泡波（NFW）出现，随后在注射EB后5.5天（“年轻”卵泡，YF；n = 155）或9.5天（“成熟”卵泡，MF；n = 145）注射前列腺素F2α（PGF(2α)；25 mg，肌肉注射）。在注射PGF(2α)后60小时（MF）和84小时（YF）进行发情检测并在12小时后进行人工授精（发情-人工授精）；在此期间未检测到发情的母牛分别在72小时和96小时接受定时人工授精（TAI）并注射GnRH。在注射PGF(2α)后的前72小时内，MF组（76.3%）表现出发情的母牛比YF组（47.7%）更多（P < 0.01），但到96小时时，YF组发情检测比例（P < 0.05）和从注射PGF(2α)到发情的间隔时间（P < 0.01）均大于MF组（分别为88.6%对76.3%，78.9 ± 0.8对57.5 ± 1.6小时）。人工授精时排卵卵泡的年龄在MF组（9.32 ± 本研究旨在评估不同类型的运动对老年人认知功能的影响。我们招募了100名年龄在65岁及以上的健康老年人，将他们随机分为三组：有氧运动组、力量训练组和对照组。有氧运动组每周进行三次，每次30分钟的中等强度有氧运动，如快走或骑自行车。力量训练组每周进行两次，每次30分钟的力量训练，包括举重和俯卧撑。对照组则不进行任何有规律的运动。在干预12周后，我们使用认知测试来评估参与者的认知功能。结果显示，有氧运动组和力量训练组的认知功能均有显著改善，而对照组的认知功能没有变化。这表明，有氧运动和力量训练都可以有效提高老年人的认知功能。
0.04天）大于YF组（6.26 ± 0.02天）（P < 0.其研究目的是探讨排卵卵泡年龄对肉牛繁殖力的影响。将两组经产（n = 171）和初产（n = 129）的产后肉牛，在同步程序（5天CO-Synch + CIDR）的最后一次促性腺激素释放激素（GnRH）后5.5天（G1组，n = 162）和6.5天（G2组，n = 138），注射苯甲酸雌二醇（EB；1 mg/500 kg体重，肌肉注射）以诱导新卵泡波（NFW）出现，随后在注射EB后5.5天（“年轻”卵泡，YF组，n = 155）或9.5天（“成熟”卵泡，MF组，n = 145）注射前列腺素F2α（PGF(2α)；25 mg，肌肉注射）。在注射PGF(2α)后60小时（MF组）和84小时（YF组）进行发情检测并在12小时后进行人工授精（发情-人工授精）；在此期间未检测到发情的母牛分别在72小时和96小时接受定时人工授精（TAI）并注射GnRH。在注射PGF(2α)后的前72小时内，MF组（76.3%）表现出发情的母牛比YF组（47.7%）更多（P < 0.01），但到96小时时，YF组发情检测比例（P < 0.05）和从注射PGF(2α)到发情的间隔时间（P < 0.01）均大于MF组（分别为88.6%对76.3%，78.9 ± 0.8对57.5 ± 1.6小时）。人工授精时排卵卵泡的年龄在MF组（9.32 ± 0.04天）大于YF组（6.26 ± 0.02天）（P < 0.01），但人工授精时卵泡直径和妊娠率在MF组（13.1 ± 0.2 mm；72.0%）和YF组（12.9 ± 0.1 mm；）之间没有差异。无论治疗如何，发情-人工授精时排卵卵泡直径和妊娠率（13.1 ± 0.1 mm；75.0%）均大于定时人工授精（12,6 ± 0.2 mm；55.4%）（P < 0.01）。在MF组中，在注射EB后但在注射PGF(2α)之前开始第二次新卵泡波的母牛（MF2组；n = 47）在72小时时用GnRH和定时人工授精诱导排卵时，排卵卵泡为4天龄，直径为10.2 ± 0.2 mm。MF2组定时人工授精的妊娠率（51.1%）与MF组和YF组定时人工授精的妊娠率（55.4%）没有差异。总之，排卵卵泡的年龄影响发情和人工授精的间隔时间，但不影响哺乳肉牛的妊娠率。
01），但人工授精时卵泡直径和妊娠率在MF组（13.1 ± 0.2 mm；72.0%）和YF组（12.9 ± 0.1 mm；67.1%）之间没有差异。无论治疗如何，发情-人工授精时排卵卵泡直径和妊娠率（13.1 ± 0.1 mm；75.0%）均大于定时人工授精（12.6 ± 0.2 mm；55.4%）（P < 0.01）。在MF组中，在注射EB后但在注射PGF(2α)之前开始第二次新卵泡波的母牛（MF2组；n = 47）在72小时时用GnRH和定时人工授精诱导排卵时，排卵卵泡为4天龄，直径为10.2 ± 0.2 mm。MF2组定时人工授精的妊娠率（51.1%）与MF组和YF组定时人工授精的妊娠率（55.4%）没有差异。总之，排卵卵泡的年龄影响发情和人工授精的间隔时间，但不影响哺乳肉牛的妊娠率。
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