[采用脑微透析和自动血液技术对大鼠丹参素的药代动力学研究]

[Pharmacokinetics study on Danshensu in rats by brain microdialysis and automated blood technique].

作者信息

Ji Lian-Ying, Yang Zhi-Hong, Hou Cong-Song, Sun Xiao-Bo

机构信息

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Nov;38(21):3758-62.

PMID:24494568

Abstract

To research the pharmacokinetic of Danshensu in brain via microdialysis method and automated blood technique. A microdialysis probe was inserted into the left lateral ventricle, and then dialysate samples and blood samples were continuously collected after iv Danshensu. LC-MS/MS was used to determinate for Danshensu in the dialysate samples. The in vivo recovery was used for the calibration of probe. WinNonlin was used for analyzing all pharmacokinetic data. Pharmacokinetic parameters of DSS in blood and in brain showed that Ke, t1/2,, AUC0-t, MRT were 0.04, 0.018 min(-1), 16.64, 58.76 min, 812.59, 51.19 min x mg x L(-1), 15.28, 79.97 min, respectively. The results were indicated that the study was successfully established LC-MS/MS detection method for Danshensu. Microdialysis combined with automated blood technique could better reflect the dynamic characteristics of Danshensu in the rat brain, and it provides a new perspective for pharmacokinetic study.

摘要

采用微透析法和自动采血技术研究丹参素在脑中的药代动力学。将微透析探针插入左侧脑室，静脉注射丹参素后连续收集透析液样本和血液样本。采用液相色谱-串联质谱法（LC-MS/MS）测定透析液样本中的丹参素。用体内回收率对探针进行校准。使用WinNonlin分析所有药代动力学数据。丹参素在血液和脑中的药代动力学参数显示，消除速率常数（Ke）、半衰期（t1/2）、零至t时间的曲线下面积（AUC0-t）、平均滞留时间（MRT）分别为0.04、0.018 min(-1)、16.64、58.76 min、812.59、51.19 min·mg·L(-1)、15.28、79.97 min。结果表明，本研究成功建立了丹参素的LC-MS/MS检测方法。微透析结合自动采血技术能更好地反映丹参素在大鼠脑中的动态特征，为药代动力学研究提供了新的视角。