Zanini Surama F, Silva-Angulo Angela B, Rosenthal Amauri, Aliaga Dolores Rodrigo, Martínez Antonio
1 Department of Veterinary Medicine, Espirito Santo Federal University , Alegre, Brazil .
Foodborne Pathog Dis. 2014 Apr;11(4):265-71. doi: 10.1089/fpd.2013.1635. Epub 2014 Feb 4.
The main goal of this work was to study the bacterial adaptive responses to antibiotics induced by sublethal concentration of citral on first-and second-generation cells of Listeria monocytogenes serovar 4b (CECT 4032) and Salmonella enterica serovar Typhimurium (CECT 443). The first-generation cells were not pretreated with citral, while the second-generation cells were obtained from cells previously exposed to citral during 5 h. The trials were conducted at 37°C. The presence of citral in the culture medium and the antibiotic strips resulted in a reduced minimum inhibitory concentration (MIC) for the first-generation cells of Listeria monocytogenes serovar 4b and Salmonella Typhimurium. This result was observed for almost all the antibiotics, compared with the same microorganisms of the control group (without citral), which could represent an additive effect. For Listeria serovar 4b, the second-generation cells of the test group maintained the same susceptibility to antibiotics compared with cells in the control group and in the test group of the first generation. The second-generation cells of the control group indicated that the Salmonella Typhimurium maintained the same sensitivity to the antibiotics tested compared with the first generation of this group, except in the case of erythromycin, which exhibited an increased MIC value. With respect to the second-generation cells of Salmonella Typhimurium, the presence of citral determined a decrease in the antibiotic susceptibility for almost all of the antibiotics, except colistin, compared with the first-generation of the test group, which can be seen by increase of MIC values. In conclusion, the presence of citral in the culture medium of Listeria 4b and Salmonella Typhimurium increased the antibiotic susceptibility of the first generations, while we observed an increase in antibiotic resistance in the second generation of Salmonella Typhimurium.
这项工作的主要目标是研究柠檬醛亚致死浓度对单核细胞增生李斯特菌血清型4b（CECT 4032）和鼠伤寒沙门氏菌（CECT 443）第一代和第二代细胞诱导产生的细菌对抗生素的适应性反应。第一代细胞未用柠檬醛预处理，而第二代细胞是从先前暴露于柠檬醛5小时的细胞中获得的。试验在37°C下进行。培养基和抗生素纸片中柠檬醛的存在导致单核细胞增生李斯特菌血清型4b和鼠伤寒沙门氏菌第一代细胞的最低抑菌浓度（MIC）降低。与对照组（无柠檬醛）的相同微生物相比，几乎所有抗生素都观察到了这一结果，这可能代表一种相加效应。对于李斯特菌血清型4b，试验组的第二代细胞与对照组和第一代试验组的细胞相比，对抗生素的敏感性保持相同。对照组的第二代细胞表明，鼠伤寒沙门氏菌与该组第一代细胞相比，对所测试抗生素的敏感性保持相同，但红霉素的MIC值有所增加除外。关于鼠伤寒沙门氏菌的第二代细胞，与试验组第一代细胞相比，柠檬醛的存在导致几乎所有抗生素（除黏菌素外）的抗生素敏感性降低，这可通过MIC值的增加看出。总之，李斯特菌4b和鼠伤寒沙门氏菌培养基中柠檬醛的存在增加了第一代细胞的抗生素敏感性，而我们观察到鼠伤寒沙门氏菌第二代细胞的抗生素耐药性增加。
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