Kovacs K, Ryan N, Stefaneanu L
Department of Pathology, St. Micheal's Hospital, University of Toronto, Ontario, Canada.
Pathol Res Pract. 1987 Dec;182(6):775-9. doi: 10.1016/s0344-0338(87)80041-9.
The presence of mAB lu-5, a panepithelial, monoclonal antibody was studied in human adenohypophyses and pituitary adenomas by immunohistochemistry using the avidinbiotin-peroxidase complex technique. In nontumorous adenohypophyses, only corticotrophs showed strong immunoreactivity, whereas other adenohypophysial cell types exhibited little or no staining. Positive immunostaining was observed in corticotrophs spreading to the posterior lobe, in cells of squamous nests located in the pars tuberalis and several cells lining pars intermedia cavities. The Crooke's hyaline material in the cytoplasm of corticotrophs was immunopositive. In adenomatous corticotrophs and cytoplasmic fibrous bodies of sparsely granulated adenomatous somatotrophs, distinct mAB lu-5 immunoreactivity was evident. GH-, PRL-, TSH-, FSH-, LH-, alpha-subunit-producing adenomas, null cell adenomas and oncocytomas showed no convincing staining. Immunopositivity in corticotroph adenomas was diffusely distributed in the cytoplasm and was not located in secretory granules, indicating that mAB lu-5 did not stain ACTH. Immunoreactivity with mAB lu-5 was not specific for pituitary corticotrophs, since the antibody stained nontumorous epithelial cells and epithelial tumor cells in other organs as well. It can be concluded that mAB lu-5 is a valuable immunocytochemical marker in pituitary related studies, especially in those pituitary adenomas in which immunostaining for ACTH is weak or equivocal; in these cases, it can confirm the diagnosis of corticotroph adenoma. The antibody yields similar results as keratin antisera. In our experience, however, it gives a stronger, more distinct immunopositivity with less nonspecific background staining.
采用抗生物素蛋白-生物素-过氧化物酶复合物技术，通过免疫组织化学方法研究了全上皮单克隆抗体mAB lu-5在人腺垂体和垂体腺瘤中的表达情况。在非肿瘤性腺垂体中，仅促肾上腺皮质激素细胞显示强免疫反应性，而其他腺垂体细胞类型染色很少或无染色。在扩散至后叶的促肾上腺皮质激素细胞、位于结节部的鳞状巢细胞以及中间部腔隙内衬的一些细胞中观察到阳性免疫染色。促肾上腺皮质激素细胞胞质中的克鲁克透明物质呈免疫阳性。在促肾上腺皮质激素腺瘤细胞和稀疏颗粒性生长激素腺瘤细胞的胞质纤维体中，明显可见mAB lu-5免疫反应性。生长激素、催乳素、促甲状腺激素、促卵泡激素、促黄体激素、α亚单位分泌型腺瘤、无功能细胞腺瘤和嗜酸性细胞瘤均未显示出令人信服的染色。促肾上腺皮质激素腺瘤中的免疫阳性弥漫分布于细胞质中，并不位于分泌颗粒中，这表明mAB lu-5不染色促肾上腺皮质激素。mAB lu-5的免疫反应性并非垂体促肾上腺皮质激素细胞所特有，因为该抗体也能染色其他器官的非肿瘤性上皮细胞和上皮肿瘤细胞。可以得出结论，mAB lu-5是垂体相关研究中有价值的免疫细胞化学标志物，尤其是在促肾上腺皮质激素免疫染色弱或不明确的垂体腺瘤中；在这些情况下，它可以确诊促肾上腺皮质激素腺瘤。该抗体产生的结果与角蛋白抗血清相似。然而，根据我们的经验，它产生的免疫阳性更强、更明显，非特异性背景染色更少。
