Tuerk C, Gauss P, Thermes C, Groebe D R, Gayle M, Guild N, Stormo G, d'Aubenton-Carafa Y, Uhlenbeck O C, Tinoco I
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1364-8. doi: 10.1073/pnas.85.5.1364.
The mRNA of bacteriophage T4 contains a strikingly abundant intercistronic hairpin. Within the 55 kilobases of known T4 sequence, the hexanucleotide sequence CTTCGG is found 13 times in the DNA strand equivalent to mRNA sequences. In 12 of those occurrences, the sequence is flanked by inverted repeats predictive of RNA hairpins with UUCG in the loop. Avian myeloblastosis virus reverse transcriptase, which can traverse hairpins of larger calculated stability, terminates efficiently at these CUUCGG hairpins. Thermal denaturation studies of model hairpins show that the loop sequence UUCG dramatically stabilizes RNA hairpins when compared to a control sequence. These data, when combined with previously described parameters of helix stability, suggest that T4 has utilized this loop sequence to optimize the stability of intercistronic hairpins. The stability of CUUCGG hairpins is also utilized in the RNAs of many organisms besides T4.
噬菌体T4的信使核糖核酸（mRNA）含有极为丰富的基因间发夹结构。在已知的55千碱基对的T4序列中，六核苷酸序列CTTCGG在与mRNA序列对应的DNA链中出现了13次。在其中12次出现中，该序列两侧是反向重复序列，预示着在环中带有UUCG的RNA发夹结构。禽成髓细胞瘤病毒逆转录酶能够穿越计算得出的稳定性更高的发夹结构，但在这些CUUCGG发夹处却有效终止。对模型发夹的热变性研究表明，与对照序列相比，环序列UUCG能显著稳定RNA发夹结构。这些数据与先前描述的螺旋稳定性参数相结合，表明T4利用了这个环序列来优化基因间发夹结构的稳定性。除T4外，许多生物体的RNA中也利用了CUUCGG发夹结构的稳定性。
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