测量系统性红斑狼疮中的干扰素α及其他细胞因子。

Measuring interferon alpha and other cytokines in SLE.

作者信息

Olferiev Mikhail, Lliguicota Mari, Kirou Kyriakos A, Crow Mary K

机构信息

Division of Rheumatology, Hospital for Special Surgery, New York, NY, USA.

出版信息

Methods Mol Biol. 2014;1134:131-50. doi: 10.1007/978-1-4939-0326-9_10.

DOI:10.1007/978-1-4939-0326-9_10

PMID:24497359

Abstract

The progression of disease in patients with systemic lupus erythematosus (SLE) is affected by production, accumulation, and actions of cytokines. Type I interferon (IFN), specifically IFN-α, is recognized as a central mediator of disease pathogenesis in SLE. We describe a functional assay to measure type I IFN activity in SLE plasma and have also measured the response of peripheral blood cells to that cytokine family. This method can be scaled to assess IFN functional activity, as well as activity and cellular response to other cytokines, in relation to cellular and serologic parameters relevant to SLE.

摘要

系统性红斑狼疮（SLE）患者疾病的进展受细胞因子的产生、积累及作用影响。I型干扰素（IFN），特别是IFN-α，被认为是SLE疾病发病机制的核心介质。我们描述了一种用于测量SLE血浆中I型干扰素活性的功能测定方法，并且还测量了外周血细胞对该细胞因子家族的反应。该方法可以扩展，以评估与SLE相关的细胞和血清学参数相关的IFN功能活性，以及对其他细胞因子的活性和细胞反应。