Department of Radiology, Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
Department of Pathology, Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
Korean J Radiol. 2014 Jan-Feb;15(1):29-36. doi: 10.3348/kjr.2014.15.1.29. Epub 2014 Jan 8.
To assess the feasibility of computed tomography (CT) lymphography using ethiodized oil for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh.
This experiment received approval from the institutional animal use and care administrative advisory committee. Twenty-three rabbits with VX2 carcinoma in the thigh underwent CT before and after (1 hour, 2 hour) peritumoral injection of 2 mL ethiodized oil. After the CT examination, sentinel nodes were identified by peritumoral injection of methylene blue and subsequently removed. The retrieved sentinel and non-sentinel lymph nodes were investigated with radiographic and pathologic examinations. Based on the comparison of CT findings with those of radiographic and pathologic examinations, the diagnostic performance of CT for sentinel node identification was assessed.
All 23 rabbits showed 53 ethiodized oil retention nodes on post-injection CT and specimen radiography, and 52 methylene blue-stained nodes at the right femoroiliac area. Of the 52 blue-stained sentinel nodes, 50 nodes demonstrated ethiodized oil retention. Thus, the sentinel node detection rate of CT was 96% (50 of 52). On pathologic examination, 28 sentinel nodes in 17 rabbits (nodes/rabbit, mean ± standard deviation, 1.7 ± 0.6) harbored metastasis. Twenty seven of the 28 metastatic sentinel nodes were found to have ethiodized oil retention.
Computed tomography lymphography using ethiodized oil may be feasible for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh.
评估使用碘油计算机断层扫描（CT）淋巴造影术对兔大腿VX2 癌实验诱导的前哨淋巴结定位的可行性。
本实验获得机构动物使用和护理行政咨询委员会的批准。23 只大腿患有 VX2 癌的兔子在瘤周注射 2 毫升碘油前后进行 CT 检查。CT 检查后，通过瘤周注射亚甲蓝识别前哨淋巴结，然后切除。对取回的前哨和非前哨淋巴结进行放射学和病理学检查。基于 CT 发现与放射学和病理学检查的比较，评估 CT 对前哨淋巴结识别的诊断性能。
所有 23 只兔子在注射后 CT 和标本放射摄影上均显示 53 个碘油保留淋巴结，在右股髂区显示 52 个亚甲蓝染色淋巴结。在 52 个蓝色染色的前哨淋巴结中，有 50 个节点显示碘油保留。因此，CT 对前哨淋巴结的检测率为 96%（50 个中的 52 个）。在病理检查中，17 只兔子的 28 个前哨淋巴结（每个兔子的淋巴结/兔子，平均值±标准差，1.7±0.6）有转移。28 个转移性前哨淋巴结中有 27 个发现有碘油保留。
使用碘油的计算机断层扫描淋巴造影术可能适用于兔大腿实验诱导的 VX2 癌前哨淋巴结定位。
