Soufizomorrod Mina, Soleimani Masoud, Hajifathali Abbas, Mohammadi Majid Mossahebi, Abroun Saeed
Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran.
Department of Medical Oncology and Hematology, Taleghani Hospital, Shahid Beheshti University (MC), Tehran, Iran.
Int J Hematol Oncol Stem Cell Res. 2013;7(1):9-14.
Umbilical cord Blood (UCB) as a source of Hematopoietic Stem/Progenitor cells (HSPCs) used for Umbilical cord blood transplantation (UCBT). The main obstacle in application of this source as an appropriate source of HSPCs is low volume of this product. So ex vivo expansion of these cells in a microenvironment which mimic body condition is important. In current study we designed biocompatible microwells in which collagene type I is coated by softlitography method. Our findings designated that in 3-Dimensional (3D) microenvironment CD133(+) UCB derived HSC expanded significantly compared to 2-Dimensional (2D) microenvironment.
脐带血（UCB）作为造血干/祖细胞（HSPCs）的来源，用于脐带血移植（UCBT）。将该来源作为合适的HSPCs来源应用的主要障碍是该产品的体积较小。因此，在模拟身体状况的微环境中对这些细胞进行体外扩增很重要。在当前研究中，我们设计了生物相容性微孔，其中通过软光刻法包被I型胶原。我们的研究结果表明，在三维（3D）微环境中，与二维（2D）微环境相比，源自脐带血的CD133(+)造血干细胞显著扩增。
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