Field experiment was conducted to evaluate the growth performance of monosex tilapia using homemade feed with Peninsula Group fish meal and commercially available feed with local fish meal in earthen mini ponds from June-September 2010. Three ponds (T1) were supplied with prepared feed and the other three ponds (T2) with commercially available fish feed. Fish were fed at the rate of 10% of their body weight for the first thirty days then gradually reduced to 6% for the next ten days, 2% for the next ten days and 3% for remaining days. The temperature were ranged from 31.5-33.0 degrees C, DO from 5.5-15 mg L(-1) in T1 and 6.5-14 mg L(-1) in T2, pH from 7.1-8.0 in T1 and 7.1-7.7 in T2, alkalinity from 105-160 mg L(-1) inT1 and 100-145 mg L(-1) in T2, nitrate was 0.06 mg L(-1) in both treatments and ammonia from 0.02 and 0.04 mg L(-1) in T1 and T2, respectively. The results of the present study showed that the best weight gain was observed as 123.48 g in T1 than T2 (111.82 g). The Specific Growth Rate (SGR) was recorded 3.09 and 2.97 and the Food Conversion Ratio (FCR) was 1.51 and 1.40 in T1 and T2, respectively. There was significant (p < 0.05) variation among the survival rate (%) of fishes which were 75.55 and 90.37% in T1 and T2, respectively. The fish productions were 19076 and 16312.11 kg ha(-1) in T1 and T2. The highest net profit (Taka/ha/70 days) of Tk. 15, 83,213 was obtained with T1 So, the prepared feed showed better performance with monosex tilapia in compared with commercial fish feed with local fish meal.