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桃组织中的赤霉素样物质:薄层层析和色谱法分离。

Gibberellin-like substances in peach tissue: Separation by thin-layer electrophoresis and chromatography.

机构信息

Fruit Research Division, D.S.I.R., Auckland, New Zealand.

出版信息

Planta. 1967 Dec;74(4):324-9. doi: 10.1007/BF00389091.

Abstract

Thin-layer electrophoresis in conjunction with thin-layer chromatography proved an excellent method for separating gibberellin-like compounds extracted from small quantities of peach tissue. Eleven compounds active in the barley-endosperm gibberellin bioassay were separated from peach shoot tips. Two of these were possibly identical with known gibberellins but the remainder were distinctly different. Of the latter, five were acidic, one was neutral, and three were basic. The most active compound in shoot tips may have been GA1 or GA3 but in seeds the most active compound resembled no known gibberellin.

摘要

薄层电泳结合薄层色谱法被证明是一种从桃组织中提取的赤霉素样化合物进行分离的极好方法。从桃梢中分离出 11 种在大麦胚乳赤霉素生物测定中具有活性的化合物。其中两种可能与已知赤霉素相同,但其余的则明显不同。在后一种化合物中,有 5 种是酸性的,1 种是中性的,3 种是碱性的。梢中最活跃的化合物可能是 GA1 或 GA3,但在种子中,最活跃的化合物与已知的赤霉素没有相似之处。

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