Bertsch A, Butin H
Institut für Forstbotanik und Forstgenetik der Universität Göttingen, Goötingen, Deutschland.
Planta. 1966 Mar;72(1):29-42. doi: 10.1007/BF00388142.
Culture experiments with the lichen Endocarpon pusillum corroborate the often doubted observations and results of STAHL (1877). It is possible to cultivate this lichen in the laboratory from spore to spore. After two months a differentiation into cortex, algae-layer, medulla and rhizines is developed and after 5-6 months the formation of mature and germinable ascospores is finished.Observations of the spore dispersal showed that the ascospores are ejected up to 5 cm. The spore dispersal can endure for some days and in this way produces a compact dissemination.The thallus development (germination of ascospores, transformation of hymenialalgae into thallus-algae, development of thallus initials, prothalli and primary thalli) and the formation of mature perithecia with germinable ascospores in laboratory cultures is described and illustrated.The opinion that such quickly growing and developing epigaeic lichens are especially suitable for laboratory cultures is emphasized and the problem of "lichensynthesis" is discussed in relation to the results of the experiments.
对地衣小内生盘菌进行的培养实验证实了施塔尔(1877年)那些常遭质疑的观察结果。在实验室里,从孢子到孢子培养这种地衣是可行的。两个月后,它分化出皮层、藻层、髓层和假根,5至6个月后,成熟且可萌发的子囊孢子形成完毕。对孢子传播的观察表明,子囊孢子可弹射至5厘米远。孢子传播可持续数天,从而形成密集的传播。本文描述并展示了在实验室培养中地衣体的发育过程(子囊孢子的萌发、子实层藻类向地衣体藻类的转变、地衣体原基、原叶体和初生地衣体的发育)以及带有可萌发子囊孢子的成熟子囊壳的形成。强调了这种快速生长和发育的地表地衣特别适合实验室培养的观点,并结合实验结果讨论了“地衣合成”问题。