Vashakidze R P, Mzhaviia N Z, Kolchinskiĭ A M, Anan'ev E V
Mol Biol (Mosk). 1988 Mar-Apr;22(2):362-8.
Clone Dm A89 was obtained upon cloning of DNA fragments coding abundant poly(A+)RNA's of D. melanogaster. Dm A89 was identified as a new transposable element using in situ hybridization with polytene chromosomes of two independent highly isogenic lines of D. melanogaster oregon RC and gt wa Dm A89 hybridizes with approximately 20 sites in each line. A portion of Dm A89 is homologous to the distal part of type I ribosomal gene insertion sequence and is highly repetitive. Two other sections of the clone have much less redundancy. The unity of the three fragments is not casual, as revealed by cloning of some other genomic sequences homologous to Dm A89. Dm A89 is actively transcribed throughout the development of D. melanogaster and produces polyadenylated RNA 1.1 kb long.
通过克隆编码黑腹果蝇丰富多聚腺苷酸化RNA的DNA片段获得了克隆体Dm A89。利用与黑腹果蝇俄勒冈RC和gt wa两个独立的高度近交系的多线染色体进行原位杂交，Dm A89被鉴定为一种新的转座元件。在每个品系中，Dm A89与大约20个位点杂交。Dm A89的一部分与I型核糖体基因插入序列的远端部分同源，并且高度重复。克隆的另外两个片段冗余度要低得多。正如通过克隆一些与Dm A89同源的其他基因组序列所揭示的那样，这三个片段的统一并非偶然。在黑腹果蝇的整个发育过程中，Dm A89都在活跃转录，并产生长度为1.1 kb的多聚腺苷酸化RNA。
