在溶菌酶存在下合成的蓝色发射铜纳米簇作为细胞标记的候选物。
Blue-emitting copper nanoclusters synthesized in the presence of lysozyme as candidates for cell labeling.
作者信息
Ghosh Rama, Sahoo Amaresh Kumar, Ghosh Siddhartha Sankar, Paul Anumita, Chattopadhyay Arun
机构信息
Department of Chemistry, ‡Centre for Nanotechnology, and §Department of Biotechnology, Indian Institute of Technology Guwahati , Guwahati-781039, India.
出版信息
ACS Appl Mater Interfaces. 2014 Mar 26;6(6):3822-8. doi: 10.1021/am500040t. Epub 2014 Mar 13.
Highly fluorescent copper nanoclusters (Cu NCs) have been synthesized using single-step reduction of copper sulfate by hydrazine in the presence of lysozyme. The fluorescence quantum yield was measured to be as high as 18%. The emission was also found to be dependent on the excitation wavelength. Mass spectrometric analyses indicated the presence of species corresponding to Cu2 to Cu9. Transmission electron microscopic analyses indicated the formation of agglomerated particles of average diameter of 2.3 nm, which were constituted of smaller particles of average diameter of 0.96 nm. They were found to be stable between pH 4 and 10 and in addition having excellent chemical and photostability. The noncytotoxic NCs were used to successfully label cervical cancer HeLa cells.
在溶菌酶存在的情况下，通过肼对硫酸铜进行一步还原，合成了高荧光铜纳米簇（Cu NCs）。测得其荧光量子产率高达18%。还发现发射光依赖于激发波长。质谱分析表明存在对应于Cu2至Cu9的物种。透射电子显微镜分析表明形成了平均直径为2.3 nm的团聚颗粒，这些颗粒由平均直径为0.96 nm的较小颗粒组成。发现它们在pH值4至10之间稳定，并且具有出色的化学稳定性和光稳定性。这些无细胞毒性的纳米簇被成功用于标记宫颈癌HeLa细胞。
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