多重耐药鲍曼不动杆菌中仅对黏菌素敏感菌株的分子流行病学研究

The molecular epidemiological study of colistin-only-sensitive strains in multi-drug resistant Acinetobacter baumannii.

作者信息

Yang Li, Han Lizhong, Sun Jingyong, Yu Yunsong, Ni Yuxing

机构信息

Nosocomial Infection Control Office of Ruijin Hospital, College of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China.

出版信息

Front Med China. 2007 Oct;1(4):423-8. doi: 10.1007/s11684-007-0083-6.

DOI:10.1007/s11684-007-0083-6

PMID:24573939

Abstract

This paper reported the epidemiology of the colistin-only-sensitive Acinetobacter baumannii (COS-AB) in a tertiary teaching hospital in China. We analyzed the clinical data of 136 COS-AB isolates from June 2004 to May 2005 and collected 66 A. baumannii isolates in which 33 strains were COS-AB, and the rest were non-COS-AB. Random amplified polymorphic DNA (RAPD) analysis (primer ERIC2 and 272) showed that all COS-AB were identical, while pulsed-field gel electrophotesis (PFGE) analysis showed two separate genotypes of these COS-AB which were distinctly different from that of non-COS-AB. The COS-AB from burn wards showed the identical PFGE pattern which was distinguished from the genotype of COS-AB in other departments, mainly surgical systems. The cross-infection was severe and strict methods of disinfection and sterilization should be implemented. Meanwhile, the epidemiology of COS-AB in environment and patients should be closely monitored. The PFGE analysis is a reliable method of A. baumannii typing.

摘要

本文报道了中国一家三级教学医院中仅对黏菌素敏感的鲍曼不动杆菌（COS-AB）的流行病学情况。我们分析了2004年6月至2005年5月期间136株COS-AB分离株的临床数据，并收集了66株鲍曼不动杆菌分离株，其中33株为COS-AB，其余为非COS-AB。随机扩增多态性DNA（RAPD）分析（引物ERIC2和272）显示所有COS-AB均相同，而脉冲场凝胶电泳（PFGE）分析显示这些COS-AB有两种不同的基因型，与非COS-AB的基因型明显不同。烧伤病房的COS-AB显示出相同的PFGE模式，与其他科室（主要是外科系统）的COS-AB基因型不同。交叉感染严重，应实施严格的消毒和灭菌方法。同时，应密切监测环境和患者中COS-AB的流行病学情况。PFGE分析是鲍曼不动杆菌分型的可靠方法。