p21激活激酶6在肝细胞癌中的表达及预后意义

Expression and prognostic significance of p21-activated kinase 6 in hepatocellular carcinoma.

作者信息

Chen Hongwei, Miao Jinlin, Li Hongchen, Wang Chunhua, Li Junliang, Zhu Yong, Wang Jianxin, Wu Xia, Qiao Hongying

机构信息

Department of Clinical Laboratory, The First Hospital of Qinhuangdao, Qinhuangdao, China.

Department of Magnetic Resonance Imaging, The Fourth People's Hospital of Taizhou, Taizhou, China.

出版信息

J Surg Res. 2014 Jun 1;189(1):81-8. doi: 10.1016/j.jss.2014.01.049. Epub 2014 Jan 29.

DOI:10.1016/j.jss.2014.01.049

PMID:24576777

Abstract

BACKGROUND

p21-activated protein kinase (PAK) 6 is a serine-threonine kinase belonging to the PAK family. Previous studies have indicated that abnormal expressions of PAK1, PAK2, and PAK5 played critical roles in hepatocellular carcinoma (HCC). Recent studies suggested that deregulation of PAK6 expression played an important role in oncogenesis. To explore the potential roles of PAK6 in HCC, expression of PAK6 was detected in human HCC specimens.

METHODS

Immunohistochemistry and Western blot analysis were performed for PAK6 in 121 HCC samples. The data were correlated with clinicopathologic features. The univariate and multivariate survival analyses were also performed to determine their clinical prognostic significance.

RESULTS

PAK6 was overexpressed in HCC as compared with the adjacent noncancerous liver tissues. High expression of PAK6 was associated with Edmondson-Steiner grade (P = 0.006) and number of tumor nodules (P < 0.001), and PAK6 was positively correlated with proliferation marker Ki-67 (P < 0.01). Univariate analysis suggested that PAK6 expression was associated with poor prognosis (P < 0.001). Multivariate analysis indicated that PAK6 and Ki-67 protein expressions were independent prognostic markers for HCC (P = 0.0245 and 0.0331, respectively).

CONCLUSIONS

Our results suggest that PAK6 overexpression is involved in the pathogenesis of HCC; it may be an independent poor prognostic factor for HCC.

摘要

背景

p21激活蛋白激酶（PAK）6是一种属于PAK家族的丝氨酸 - 苏氨酸激酶。先前的研究表明，PAK1、PAK2和PAK5的异常表达在肝细胞癌（HCC）中起关键作用。最近的研究表明，PAK6表达失调在肿瘤发生中起重要作用。为了探讨PAK6在HCC中的潜在作用，检测了人HCC标本中PAK6的表达。

方法

对121例HCC样本进行PAK6的免疫组织化学和蛋白质印迹分析。将数据与临床病理特征相关联。还进行了单因素和多因素生存分析以确定它们的临床预后意义。

结果

与相邻的非癌性肝组织相比，PAK6在HCC中过表达。PAK6的高表达与Edmondson-Steiner分级（P = 0.006）和肿瘤结节数量（P < 0.001）相关，并且PAK6与增殖标志物Ki-67呈正相关（P < 0.01）。单因素分析表明，PAK6表达与预后不良相关（P < 0.001）。多因素分析表明，PAK6和Ki-67蛋白表达是HCC的独立预后标志物（分别为P = 0.0245和0.0331）。