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外周感觉神经失活小鼠模型中骨骼发育的改变。

Altered bone development in a mouse model of peripheral sensory nerve inactivation.

作者信息

Heffner M A, Anderson M J, Yeh G C, Genetos D C, Christiansen B A

机构信息

University of California-Davis Medical Center, Department of Orthopaedic Surgery.

出版信息

J Musculoskelet Neuronal Interact. 2014 Mar;14(1):1-9.

Abstract

OBJECTIVES

The present study sought to determine the effects of decreased peripheral sensory nerve function on skeletal development and bone metabolism in mice.

METHODS

C57BL/6 neonatal mice were treated with capsaicin to induce peripheral sensory nerve degeneration, and compared to vehicle-treated controls at 4, 8 and 12 weeks of age. Changes in bone structure were assessed using micro-computed tomography, mechanical properties and fracture resistance were assessed using three-point bending of radii, and bone turnover was assessed using dynamic histomorphometry and serum biomarkers.

RESULTS

Capsaicin treatment resulted in small but significant decreases in bone structure, particularly affecting trabecular bone. Capsaicin-treated mice exhibited lower trabecular thickness at the femoral metaphysis and L5 vertebral body compared with vehicle-treated mice. However, capsaicin- and vehicle-treated mice had similar mechanical properties and bone turnover rates.

CONCLUSION

Neonatal capsaicin treatment affected trabecular bone during development; however these small changes may not be meaningful with respect to bone strength under normal loading conditions. It is possible that capsaicin-sensitive neurons may be more important for bone under stress conditions such as increased mechanical loading or injury. Future studies will investigate this potential role of peripheral sensory nerves in bone adaptation.

摘要

目的

本研究旨在确定外周感觉神经功能降低对小鼠骨骼发育和骨代谢的影响。

方法

用辣椒素处理C57BL/6新生小鼠以诱导外周感觉神经变性,并在4周、8周和12周龄时与用赋形剂处理的对照小鼠进行比较。使用微型计算机断层扫描评估骨结构变化,使用桡骨三点弯曲评估力学性能和抗骨折能力,使用动态组织形态计量学和血清生物标志物评估骨转换。

结果

辣椒素处理导致骨结构出现虽小但显著的下降,尤其影响小梁骨。与用赋形剂处理的小鼠相比,用辣椒素处理的小鼠在股骨干骺端和L5椎体处的小梁厚度较低。然而,用辣椒素处理的小鼠和用赋形剂处理的小鼠具有相似的力学性能和骨转换率。

结论

新生小鼠辣椒素处理在发育过程中影响小梁骨;然而,在正常负荷条件下,这些微小变化可能对骨强度没有意义。在诸如机械负荷增加或受伤等应激条件下,辣椒素敏感神经元对骨可能更为重要。未来的研究将探讨外周感觉神经在骨适应中的这一潜在作用。

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本文引用的文献

1
Clinical role of bisphosphonate therapy.
Int J Womens Health. 2012;4:455-69. doi: 10.2147/IJWH.S24783. Epub 2012 Sep 3.
2
The Involvement of TRP Channels in Bone Homeostasis.
Front Endocrinol (Lausanne). 2012 Aug 20;3:99. doi: 10.3389/fendo.2012.00099. eCollection 2012.
4
Bisphosphonates: the first 40 years.
Bone. 2011 Jul;49(1):2-19. doi: 10.1016/j.bone.2011.04.022. Epub 2011 May 1.
5
Anabolic agents and bone quality.
Clin Orthop Relat Res. 2011 Aug;469(8):2215-24. doi: 10.1007/s11999-010-1722-9.
6
BoneJ: Free and extensible bone image analysis in ImageJ.
Bone. 2010 Dec;47(6):1076-9. doi: 10.1016/j.bone.2010.08.023. Epub 2010 Sep 15.
7
Effects of capsaicin-induced sensory denervation on bone metabolism in adult rats.
Bone. 2010 Jun;46(6):1591-6. doi: 10.1016/j.bone.2010.02.022. Epub 2010 Mar 1.
8
Capsaicin-sensitive sensory nerve fibers contribute to the generation and maintenance of skeletal fracture pain.
Neuroscience. 2009 Sep 15;162(4):1244-54. doi: 10.1016/j.neuroscience.2009.05.065. Epub 2009 May 29.
10
Expression of transient receptor potential (TRP) channels in human and murine osteoblast-like cells.
Mol Membr Biol. 2009 Apr;26(3):146-58. doi: 10.1080/09687680802612721. Epub 2008 Dec 27.

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