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1
Half of the (Na+ + K+)-transporting-ATPase-associated K+-stimulated p-nitrophenyl phosphatase activity of gastric epithelial cells is exposed to the surface exterior.胃上皮细胞中与（Na⁺ + K⁺）转运ATP酶相关的钾离子刺激的对硝基苯磷酸酶活性的一半暴露于细胞表面外侧。
Biochem J. 1988 May 15;252(1):29-34. doi: 10.1042/bj2520029.
2
K+-stimulated p-nitrophenyl phosphatase is not a partial reaction of the gastric (H+ + K+)-transporting ATPase. Evidence supporting a new model for the univalent-cation-transporting ATPase systems.钾离子刺激的对硝基苯磷酸酶不是胃（氢离子 + 钾离子）转运ATP酶的部分反应。支持单价阳离子转运ATP酶系统新模型的证据。
Biochem J. 1986 Jan 1;233(1):231-8. doi: 10.1042/bj2330231.
3
Properties of potassium activated p-nitrophenyl phosphatase of plasma membranes isolated from rat stomach muscle.从大鼠胃肌中分离出的质膜钾激活对硝基苯磷酸酶的性质
J Bioenerg Biomembr. 1986 Aug;18(4):295-306. doi: 10.1007/BF00743050.
4
On the ouabain-sensitive potassium activated p-nitrophenyl phosphatase activity of vascular muscle plasma membranes.关于哇巴因敏感的血管平滑肌质膜钾激活对硝基苯磷酸酶活性
Arch Int Pharmacodyn Ther. 1984 Dec;272(2):245-55.
5
Interaction of Ca2+ with (Na+ + K+)-ATPase: properties of the Ca2+-stimulated phosphatase activity.Ca2+ 与（Na+ + K+）-ATP 酶的相互作用：Ca2+ 刺激的磷酸酶活性特性
Arch Biochem Biophys. 1984 Jun;231(2):287-92. doi: 10.1016/0003-9861(84)90390-4.
6
Effect of high ionic strength and inhibitors of H,K-ATPase on the ouabain-sensitive K-p-nitrophenylphosphatase activity in the sea anemone Stichodactyla helianthus.高离子强度和H，K-ATP酶抑制剂对海葵（Stichodactyla helianthus）哇巴因敏感的K-对硝基苯磷酸酶活性的影响。
Comp Biochem Physiol B Biochem Mol Biol. 1997 Jun;117(2):217-24. doi: 10.1016/s0305-0491(96)00319-7.
7
Rat brain (Na+-K+)ATPase: modulation of its ouabain-sensitive K+-PNPPase activity by thimerosal.大鼠脑（钠钾）ATP酶：硫柳汞对其哇巴因敏感的钾-对硝基苯磷酸酶活性的调节
Int J Biochem. 1983;15(1):5-7. doi: 10.1016/0020-711x(83)90003-4.
8
Modified cation activation of the (Na+K)-ATPase following treatment with thimerosal.硫柳汞处理后（钠钾）-ATP酶的修饰阳离子激活作用
Arch Biochem Biophys. 1985 Mar;237(2):386-95. doi: 10.1016/0003-9861(85)90290-5.
9
Stimulation of p-nitrophenylphosphatase activity of Na+/K+-ATPase by NaCl with oligomycin or ATP.在存在寡霉素或ATP的情况下，NaCl对Na⁺/K⁺-ATP酶的对硝基苯磷酸酶活性的刺激作用。
FEBS J. 2005 Feb;272(3):673-84. doi: 10.1111/j.1742-4658.2004.04496.x.
10
Detection of ouabain-insensitive H(+)-transporting, K(+)-stimulated p-nitrophenylphosphatase activity in rat gastric glands by cerium-based cytochemistry.采用铈基细胞化学法检测大鼠胃腺中哇巴因不敏感的H(+)转运、K(+)刺激的对硝基苯磷酸酶活性。
J Histochem Cytochem. 1990 Dec;38(12):1895-905. doi: 10.1177/38.12.2174937.

引用本文的文献

1
Demonstration of an endogenous activator for the Na+, K(+)-ATPase system.钠钾ATP酶系统内源性激活剂的证明。
Mol Cell Biochem. 1989;91(1-2):123-9. doi: 10.1007/BF00228087.
2
Ultrastructural and cytochemical analysis of Na+, K+, ATPase and H+, K+, ATPase in parietal cells of gastric mucosa in the rabbit.兔胃黏膜壁细胞中Na+、K+ - ATP酶和H+、K+ - ATP酶的超微结构及细胞化学分析
Histochemistry. 1992;97(3):255-61. doi: 10.1007/BF00267636.

本文引用的文献

1
ENZYMATIC BASIS FOR ACTIVE TRANSPORT OF NA+ AND K+ ACROSS CELL MEMBRANE.钠离子和钾离子跨细胞膜主动运输的酶学基础
Physiol Rev. 1965 Jul;45:596-617. doi: 10.1152/physrev.1965.45.3.596.
2
Polyamines are inhibitors of gastric acid secretion.多胺是胃酸分泌的抑制剂。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1448-52. doi: 10.1073/pnas.79.5.1448.
3
Chloride-activated passive potassium transport in human erythrocytes.人红细胞中氯离子激活的被动钾转运
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1711-5. doi: 10.1073/pnas.77.3.1711.
4
Control of NaCl transport in the thick ascending limb.髓袢升支粗段中氯化钠转运的调控。
Am J Physiol. 1984 Jun;246(6 Pt 2):F745-56. doi: 10.1152/ajprenal.1984.246.6.F745.
5
Immunochemical characterization of a functional site of (Na+,K+)-ATPase.（钠钾）-ATP酶功能位点的免疫化学特性
Biochemistry. 1984 May 8;23(10):2275-81. doi: 10.1021/bi00305a029.
6
Studies on (K+ + H+)-ATPase. VI. Determination on the molecular size by radiation inactivation analysis.（钾离子 + 氢离子）-ATP 酶的研究。VI. 用辐射失活分析法测定分子大小。
Biochim Biophys Acta. 1983 Jun 10;731(2):329-37. doi: 10.1016/0005-2736(83)90025-1.
7
Cellular and subcellular aspects of the mechanism of gastric acid secretion.胃酸分泌机制的细胞及亚细胞层面
J Surg Res. 1981 Dec;31(6):496-505. doi: 10.1016/0022-4804(81)90188-8.
8
Effects of barium on gastric microsomal K+-stimulated para-nitrophenyl phosphatase activity.钡对胃微粒体钾离子刺激的对硝基苯磷酸酶活性的影响。
Can J Physiol Pharmacol. 1980 Oct;58(10):1189-91. doi: 10.1139/y80-181.
9
Isolation and viability of gastric mucosal surface cells of the rabbit.兔胃黏膜表面细胞的分离与活力
J Surg Res. 1982 Oct;33(4):265-79. doi: 10.1016/0022-4804(82)90039-7.
10
Effects of ATP and protons on the Na : K selectivity of the (Na+ + K+)-ATPase studied by ligand effects on intrinsic and extrinsic fluorescence.通过配体对内在和外在荧光的影响研究ATP和质子对（Na⁺ + K⁺）-ATP酶的Na⁺:K⁺选择性的影响。
Biochim Biophys Acta. 1980 Sep 18;601(2):386-402. doi: 10.1016/0005-2736(80)90543-x.

胃上皮细胞中与（Na⁺ + K⁺）转运ATP酶相关的钾离子刺激的对硝基苯磷酸酶活性的一半暴露于细胞表面外侧。

Half of the (Na+ + K+)-transporting-ATPase-associated K+-stimulated p-nitrophenyl phosphatase activity of gastric epithelial cells is exposed to the surface exterior.

作者信息

Nandi J, Das P K, Levine R A, Ray T K

机构信息

Department of Surgery, State University, New York Health Science Center, Syracuse 13210.

出版信息

Biochem J. 1988 May 15;252(1):29-34. doi: 10.1042/bj2520029.

DOI:10.1042/bj2520029
PMID:2458713
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149102/
Abstract

Ouabain inhibited 86RbCl uptake by 80% in rabbit gastric superficial epithelial cells (SEC), revealing the presence of a functional Na+,K+-ATPase [(Na+ + K+)-transporting ATPase] pump. Intact SEC were used to study the ouabain-sensitive Na+,K+-ATPase and K+-pNPPase (K+-stimulated p-nitrophenyl phosphatase) activities before and after lysis. Intact SEC showed no Na+,K+-ATPase and insignificant Mg2+-ATPase activity. However, appreciable K+-pNPPase activity sensitive to ouabain inhibition was demonstrated by localizing its activity to the cell-surface exterior. The lysed SEC, on the other hand, demonstrated both ouabain-sensitive Na+,K+-ATPase and K+-pNPPase activities. Thus the ATP-hydrolytic site of Na+,K+-ATPase faces exclusively the cytosol, whereas the associated K+-pNPPase is distributed equally across the plasma membrane. The study suggests that the cell-exterior-located K+-pNPPase can be used as a convenient and reliable 'in situ' marker for the functional Na+,K+-ATPase system of various isolated cells under noninvasive conditions.

摘要

哇巴因抑制兔胃表层上皮细胞（SEC）对86RbCl的摄取达80%，这表明存在功能性钠钾ATP酶[（钠 + 钾）转运ATP酶]泵。完整的SEC用于研究裂解前后对哇巴因敏感的钠钾ATP酶和钾依赖对硝基苯磷酸酶（K + 刺激的对硝基苯磷酸酶）活性。完整的SEC未显示钠钾ATP酶活性，且镁离子ATP酶活性不显著。然而，通过将其活性定位到细胞表面外侧，证实了存在对哇巴因抑制敏感的可观的钾依赖对硝基苯磷酸酶活性。另一方面，裂解后的SEC显示出对哇巴因敏感的钠钾ATP酶和钾依赖对硝基苯磷酸酶活性。因此，钠钾ATP酶的ATP水解位点仅面向细胞质，而相关的钾依赖对硝基苯磷酸酶则均匀分布于质膜。该研究表明，位于细胞外侧的钾依赖对硝基苯磷酸酶可作为一种方便可靠的“原位”标记物，用于在非侵入性条件下研究各种分离细胞的功能性钠钾ATP酶系统。