Sakai K, Ma X Y, Volsky D J
Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital Center, College of Physicians & Surgeons, Columbia University, New York, NY 10019.
FEBS Lett. 1988 Oct 10;238(2):257-61. doi: 10.1016/0014-5793(88)80491-5.
Single genotypic variants of HIV-I, contained in a parental cytopathic HIV-I isolate, were isolated by molecular cloning and propagated in susceptible cells. Two such HIV-I clones, designated N1T-E and N1T-A, exhibited similar restriction endonuclease maps but strikingly different biological activities. Infection of T lymphocytes or monocytes by clone N1T-E was characterized by slow kinetics and lack of significant cytopathic effects, but high reverse transcriptase activity levels in culture supernatants of chronically-infected cells. Clone N1T-A, like the parental HIV-I isolate, exhibited fast kinetics of infection in T cells and monocytes and strong cytopathicity in these cells. Full characterization of the low-cytopathic virus in comparison to the structurally similar cytopathic clone may facilitate the elucidation of the molecular basis of HIV cytopathogenicity.
从一种具有细胞病变效应的HIV-1亲代分离株中分离出单个基因型变体，通过分子克隆进行分离，并在易感细胞中进行繁殖。两个这样的HIV-1克隆，命名为N1T-E和N1T-A，表现出相似的限制性内切酶图谱，但生物学活性却显著不同。克隆N1T-E感染T淋巴细胞或单核细胞的特点是动力学缓慢且缺乏明显的细胞病变效应，但在慢性感染细胞的培养上清液中逆转录酶活性水平较高。克隆N1T-A与HIV-1亲代分离株一样，在T细胞和单核细胞中表现出快速的感染动力学以及在这些细胞中的强细胞病变效应。与结构相似的具有细胞病变效应的克隆相比，对低细胞病变病毒进行全面表征可能有助于阐明HIV细胞致病性的分子基础。
