Yang L M, Liu Y, Zhao J, Hao L M, Huang K X, Jiang W H
The First Affiliated Hospital of Jilin University Department of Nephropathy Changchun China.
Norman Bethune Medical College of Jilin University Department of Histology and Embryology Changchun China.
Cell Mol Biol (Noisy-le-grand). 2014 Mar 5;60(1):12-8.
The human umbilical cord represents a promising resource of mesenchymal stem cells (MSCs). In order to improve our understanding of MSCs derived from human umbilical cord (UC-MSCs), we isolated UC-MSCs from human umbilical cord tissues through a direct culture approach. We performed a comprehensive characterization of these cells based on analyses of morphology, growth features, cell surface antigen markers and differentiation capacity. All these analyses validated their stem cell nature. The UC-MSCs presented a spindle-shaped morphology and could be subcultured for up to 15 passages without losing their cellular features. Moreover, these UC-SMCs presented an expression profile of cell surface antigens similar to other MSCs: positive for CD44, CD90, and CD105 expression and negative for CD34, CD31, and CD45 expression. Differentiation assays further validated the multipotency of UC-MSCs by inducing these cells into osteoblasts, adipocytes and functional hepatocytes. Our studies clearly demonstrated that UC-MSCs resemble other types of MSCs in many aspects and have a great potential to be applied in tissue engineering and regenerative medicine.
人脐带是间充质干细胞（MSCs）的一种有前景的来源。为了增进我们对源自人脐带的间充质干细胞（UC-MSCs）的了解，我们通过直接培养法从人脐带组织中分离出UC-MSCs。我们基于形态学、生长特性、细胞表面抗原标志物及分化能力分析对这些细胞进行了全面表征。所有这些分析均证实了它们的干细胞特性。UC-MSCs呈现出纺锤形形态，并且可以传代培养多达15代而不丧失其细胞特征。此外，这些UC-SMCs呈现出与其他MSCs相似的细胞表面抗原表达谱：CD44、CD90和CD105表达呈阳性，而CD34、CD31和CD45表达呈阴性。分化试验通过将这些细胞诱导分化为成骨细胞、脂肪细胞和功能性肝细胞，进一步证实了UC-MSCs的多能性。我们的研究清楚地表明，UC-MSCs在许多方面类似于其他类型的MSCs，并且在组织工程和再生医学中具有巨大的应用潜力。
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