利用分子印迹聚合物包覆的 CdTe 量子点对克伦特罗和三聚氰胺的选择性光传感。
Selective optosensing of clenbuterol and melamine using molecularly imprinted polymer-capped CdTe quantum dots.
机构信息
Department of Chemistry, Changwon National University, Changwon 641-773, South Korea; Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology (VAST), 2 Hung Vuong, Nhatrang, Vietnam.
Department of Chemistry, Changwon National University, Changwon 641-773, South Korea.
出版信息
Biosens Bioelectron. 2014 Jul 15;57:310-6. doi: 10.1016/j.bios.2014.02.041. Epub 2014 Feb 25.
A novel procedure for the optosensing of clenbuterol and melamine was developed using molecularly imprinted polymer-capped CdTe quantum dots (MIP-CdTe QDs). The MIP-CdTe QDs were synthesized by a radical polymerization process among CdTe QDs, a template, 3-aminopropyltriethoxysilane (APTES) and tetraethoxysilane (TEOS). The sizes of the MIP-CdTe particles were controlled by the speed of polymerization, concentration of the template, concentration of the quantum dots, and the ratio of template, monomer and cross-linker. Excellent selectivity and high sensitivity of MIP-CdTe QDs toward clenbuterol/melamine molecules were observed based on the fluorescence quenching of QDs. Experimental results showed that the optimum molar ratios of template, monomer, and cross-linker were 1:8:20 and 1:4:20 for analyzing clenbuterol and melamine, respectively. Under optimum conditions, these MIP-CdTe QDs showed a limit of detection of 0.4 μM (120 ng/mL) for clenbuterol and 0.6 μM (75 ng/mL) for melamine. The feasibility of the developed method in real samples was successfully evaluated through the analysis of clenbuterol and melamine in milk and liver samples with satisfactory recoveries of 92-97%. The MIP-CdTe QDs could be easily regenerated for subsequent sample analysis with water.
一种使用分子印迹聚合物包覆的 CdTe 量子点（MIP-CdTe QDs）对克伦特罗和三聚氰胺进行光传感的新方法已经开发出来。MIP-CdTe QDs 是通过 CdTe QDs、模板、3-氨丙基三乙氧基硅烷（APTES）和四乙氧基硅烷（TEOS）之间的自由基聚合过程合成的。MIP-CdTe 颗粒的大小可以通过聚合速度、模板浓度、量子点浓度以及模板、单体和交联剂的比例来控制。基于 QDs 的荧光猝灭，观察到 MIP-CdTe QDs 对克伦特罗/三聚氰胺分子具有优异的选择性和高灵敏度。实验结果表明，分析克伦特罗和三聚氰胺的最佳模板、单体和交联剂的摩尔比分别为 1:8:20 和 1:4:20。在最佳条件下，这些 MIP-CdTe QDs 对克伦特罗的检测限为 0.4 μM（120 ng/mL），对三聚氰胺的检测限为 0.6 μM（75 ng/mL）。通过对牛奶和肝脏样品中克伦特罗和三聚氰胺的分析，成功评估了该方法在实际样品中的可行性，回收率为 92-97%。MIP-CdTe QDs 可以很容易地用水再生，用于后续的样品分析。
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