天然水蛭素局部应用对猪模型随意型皮瓣微循环的影响。

The effect of local application of natural hirudin on random pattern skin flap microcirculation in a porcine model.

作者信息

Yin Guo-Qian, Sun Zhi-Yong, Wang Gang

机构信息

Department of Plastic Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, 530021, Guangxi, China,

出版信息

Cell Biochem Biophys. 2014 Jul;69(3):741-6. doi: 10.1007/s12013-014-9861-3.

DOI:10.1007/s12013-014-9861-3

PMID:24633455

Abstract

The aim of this study was to investigate the effect of local administration of hirudin in improving random pattern skin flap microcirculation in a porcine model. Five Chinese minipigs were used and six dorsal random pattern skin flaps were elevated in each animal (4 × 14 cm). All flaps (n = 30) were assigned to experimental (n = 10), control (n = 10), and sham (n = 10) groups. Flap edema measurement showed that edema in experimental flaps was more severe (P < 0.05) than either control or sham flaps. Local blood flow detection showed an increased image signal of blood flow in experimental flaps instead of an obvious avascular area in control and sham flaps. The survival area was significantly greater in experimental group (67.6 ± 2.1 %) as compared to control (45.2 ± 1.4 %) or sham (48.3 ± 1.1 %) group (P < 0.05). Our data showed that local administration of hirudin can significantly improve random pattern skin flap microcirculation in over dimensioned random pattern skin flaps in a porcine model.

摘要

本研究的目的是在猪模型中研究局部应用水蛭素对改善随意型皮瓣微循环的作用。使用了5只中国小型猪，每只动物制备6个背部随意型皮瓣（4×14 cm）。所有皮瓣（n = 30）分为实验组（n = 10）、对照组（n = 10）和假手术组（n = 10）。皮瓣水肿测量显示，实验组皮瓣的水肿比对照组或假手术组更严重（P < 0.05）。局部血流检测显示，实验组皮瓣的血流图像信号增加，而对照组和假手术组皮瓣没有明显的无血管区。与对照组（45.2±1.4%）或假手术组（48.3±1.1%）相比，实验组的存活面积显著更大（67.6±2.1%）（P < 0.05）。我们的数据表明，在猪模型中，局部应用水蛭素可显著改善超尺寸随意型皮瓣中的随意型皮瓣微循环。