一种用于漂白皮肤中神经纤维的简单、可靠且廉价的银染法。
A simple, reliable and inexpensive silver stain for nerve fibers in bleached skin.
作者信息
Fritzsch B, Zakon H H
机构信息
Department of Zoology, University of Texas at Austin.
出版信息
Z Naturforsch C J Biosci. 1988 Jul-Aug;43(7-8):606-8. doi: 10.1515/znc-1988-7-820.
A procedure for silver staining is described which leads to the selective and reliable impregnation of nerve fibers in bleached skin of vertebrates and invertebrates. In combination with osmium, the protocol enhances the staining of secondary sensory cells of mechanosensory and electrosensory organs so that the innervation pattern of each organ and the number of sensory cells per organ can easily be evaluated. The technique can be also used for staining nerve fibers in whole embryos.
本文描述了一种银染程序，该程序可对脊椎动物和无脊椎动物漂白皮肤中的神经纤维进行选择性且可靠的浸染。与锇结合使用时，该方案可增强机械感觉器官和电感觉器官的次级感觉细胞的染色效果，从而能够轻松评估每个器官的神经支配模式以及每个器官的感觉细胞数量。该技术还可用于对整个胚胎中的神经纤维进行染色。
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