DNMTs和MBD2在胃肠道间质瘤中的表达
Expression of DNMTs and MBD2 in GIST.
作者信息
He Miao, Fan Jing, Jiang Rong, Tang Wei-Xue, Wang Zi-Wei
机构信息
Departments of General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China ;
Emergency, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China ;
出版信息
Biomed Rep. 2013 Mar;1(2):223-227. doi: 10.3892/br.2012.34. Epub 2012 Oct 29.
The aim of this study was to investigate the protein expression of DNA methyltransferases (DNMTs, including DNMT1, DNMT2, DNMT3A, DNMT3B and DNMT3L) and methyl-CpG-binding domain protein 2 (MBD2) in gastrointestinal stromal tumor (GIST). Immunohistochemistry and western blot analysis were used to detect expression of DNMT and MBD2 in 15 pairs of adult GIST and matched non-tumor tissues. The protein expression of DNMT1, DNMT2, DNMT3B, DNMT3L and MBD2 was significantly higher in adult GISTs compared to the matched non-tumor tissues (P<0.05). However, no significant difference was detected in the protein expression of DNMT3A between tumor and non-tumor tissues (P>0.05). Associations between DNMT1 expression and mitotic index, DNMT3B expression and tumor size, as well as DNMT3L expression and infection were detected in GISTs (P<0.05). In conclusion, GISTs exhibit a high protein expression of DNMT (with the exception of DNMT3A) and MBD2.
本研究旨在调查DNA甲基转移酶（DNMT，包括DNMT1、DNMT2、DNMT3A、DNMT3B和DNMT3L）和甲基化CpG结合域蛋白2（MBD2）在胃肠道间质瘤（GIST）中的蛋白表达情况。采用免疫组织化学和蛋白质印迹分析检测15对成人GIST及配对的非肿瘤组织中DNMT和MBD2的表达。与配对的非肿瘤组织相比，成人GIST中DNMT1、DNMT2、DNMT3B、DNMT3L和MBD2的蛋白表达显著更高（P<0.05）。然而，肿瘤组织和非肿瘤组织之间DNMT3A的蛋白表达未检测到显著差异（P>0.05）。在GIST中检测到DNMT1表达与有丝分裂指数、DNMT3B表达与肿瘤大小以及DNMT3L表达与感染之间存在关联（P<0.05）。总之，GIST表现出较高的DNMT（DNMT3A除外）和MBD2蛋白表达。
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