Yang Jin-Hu, Ren Feng
Department of General Surgery, The Fourth People's Hospital of Wuxi, Wuxi, Jiangsu 214062, P.R. China.
Department of Clinical Laboratory, The Fourth People's Hospital of Wuxi, Wuxi, Jiangsu 214062, P.R. China.
Biomed Rep. 2014 Jan;2(1):132-136. doi: 10.3892/br.2013.195. Epub 2013 Nov 4.
Acute coronary syndrome (ACS) typically occurs when coronary artery disease results in the obstruction of the coronary arteries. Tenascin-C (TNC) and OX40 ligand (OX40L) were shown to be involved in the pathogenesis of atherosclerosis. In this study, 50 healthy controls and 170 patients, including 50 patients with stable angina (SA), 70 with unstable angina and 50 with acute myocardial infarction, were evaluated to assess serum TNC and plasma OX40L levels. The serum TNC levels were measured by a quantitative automated particle-enhanced immunonephelometric assay. ELISA was used to determine the expression levels of OX40L. All the coronary stenoses with a ≥30% diameter reduction were assessed by angiographic coronary stenosis morphology. The patients with ACS exhibited a significant increase in TNC expression levels (39.39±19.80 ng/ml) compared to the levels in the control and SA groups (28.65±12.32 ng/ml, P<0.01 and 31.22±18.92 ng/ml, P<0.05, respectively). The levels of OX40L were also found to be higher in patients with ACS (38.59±15.76 ng/ml) compared to those in the control and SA groups (19.42±11.19 ng/ml, P<0.001 and 21.52±10.30 ng/ml, P<0.001, respectively). The TNC and OX40L levels were positively correlated with each other (r=0.68; P<0.001) and with fibrinogen levels (r=0.76 and r=0.45, respectively; P<0.001). A positive correlation was also observed between the expression of TNC and OX40L and complex coronary stenosis (r=0.69 and r=0.55, respectively; P<0.001). We concluded that TNC and OX40L may act synergistically in coronary plaque formation and may be also be involved in the pathogenesis of coronary lesions. Patients with ACS exhibited increased TNC and OX40L expression levels, which may have created a prothrombotic milieu, aggravating the development of atherosclerosis and the instability of atherosclerotic plaques. Therefore, the expression of TNC and OX40L may be a valuable marker for predicting the severity of ACS.
急性冠状动脉综合征（ACS）通常在冠状动脉疾病导致冠状动脉阻塞时发生。已表明腱生蛋白-C（TNC）和OX40配体（OX40L）参与动脉粥样硬化的发病机制。在本研究中，对50名健康对照者和170例患者进行了评估，其中包括50例稳定型心绞痛（SA）患者、70例不稳定型心绞痛患者和50例急性心肌梗死患者，以测定血清TNC和血浆OX40L水平。血清TNC水平通过定量自动化颗粒增强免疫比浊法测定。采用酶联免疫吸附测定法（ELISA）测定OX40L的表达水平。所有直径缩小≥30%的冠状动脉狭窄均通过血管造影冠状动脉狭窄形态进行评估。与对照组和SA组（分别为28.65±12.32 ng/ml，P<0.01和31.22±18.92 ng/ml，P<0.05）相比，ACS患者的TNC表达水平显著升高（39.39±19.80 ng/ml）。还发现ACS患者的OX40L水平（38.59±15.76 ng/ml）高于对照组和SA组（分别为19.42±11.19 ng/ml，P<0.001和21.52±10.30 ng/ml，P<0.001）。TNC和OX40L水平彼此呈正相关（r=0.68；P<0.001），且与纤维蛋白原水平呈正相关（分别为r=0.76和r=0.45；P<0.001）。TNC和OX40L的表达与复杂冠状动脉狭窄之间也呈正相关（分别为r=0.69和r=0.55；P<0.001）。我们得出结论，TNC和OX40L可能在冠状动脉斑块形成中协同作用，也可能参与冠状动脉病变的发病机制。ACS患者的TNC和OX40L表达水平升高，这可能营造了一种促血栓形成的环境，加重动脉粥样硬化的发展和动脉粥样硬化斑块的不稳定性。因此，TNC和OX40L的表达可能是预测ACS严重程度的一个有价值的标志物。
