用于同时检测抗Torque teno sus病毒1型（TTSuV1）、TTSuV2以及猪繁殖与呼吸综合征病毒1型和2型IgG抗体的4重抗体检测方法的开发与验证
Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2.
作者信息
Giménez-Lirola Luis G, Gerber Priscilla F, Rowland Raymond R, Halbur Patrick G, Huang Yao-Wei, Meng Xiang-Jin, Opriessnig Tanja
机构信息
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, USA.
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, USA; Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
出版信息
Res Vet Sci. 2014 Jun;96(3):543-50. doi: 10.1016/j.rvsc.2014.02.014. Epub 2014 Feb 27.
A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem.
开发了一种基于荧光微珠的免疫分析方法（FMIA），用于同时检测抗猪圆环病毒1型（TTSuV1）、TTSuV2、猪繁殖与呼吸综合征病毒1型（PRRSV-1）和PRRSV-2的IgG抗体。随时间从20头猪采集血清样本。20头猪中有12头在第0天接触TTSuV2，20头猪在第35天接种PRRSV-2疫苗，20头猪在第63天接触PRRSV-2。第0天，30%的猪检测到抗TTSuV抗体，到第35天，这一比例为90%。所有接种PRRSV-2疫苗的猪在接种后21天均可检测到抗PRRSV-2 IgG。还对来自17个猪场的现场样本进行了检测。PRRSV和TTSuV的血清阳性率均随年龄增长而升高。将PRRSV-2的FMIA与ELISA进行比较，结果显示在仔猪中相关性良好，但在老龄猪中假阳性率较高。PRRSV各型之间的交叉反应是一个问题。
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