Qiu Juanjuan, Ma Yufang, Owusu Lawrence, Jiang Tao, Xin Yi
Centralab, the First Affiliated Hospital of Dalian Medical University, Dalian, China.
J Basic Microbiol. 2014 Jul;54(7):670-7. doi: 10.1002/jobm.201300858. Epub 2014 Mar 20.
Serine acetyltransferase (CysE) is involved in L-cysteine biosynthesis in Mycobacterium, and it is important for the self-defense mechanism of the bacteria. Mycobacterium tuberculosis CysE (Rv2335) has been identified as a serine acetyltransferase, and it is orthologous to Mycobacterium smegmatis MSMEG_5947. In this study, the MSMEG_5947 gene was cloned, expressed, and identified as a serine acetyltransferase. To investigate the function of M. smegmatis CysE, a MSMEG_5947 knockout mutant strain (M. sm-ΔM_5947) was generated through homologous recombination. The growth and morphological characteristics of this strain were studied using growth curves and electron microscopy, respectively. M. sm-ΔM_5947 grew slower than M. smegmatis mc(2) 155. Electron microscopy revealed that the lack of the M. smegmatis CysE protein caused drastic morphological changes. Therefore, deletion of the serine acetyltransferase retards the growth of the Mycobacterium, but serine acetyltransferase expression is not essential for the survival of the bacteria.
丝氨酸乙酰转移酶（CysE）参与分枝杆菌中L-半胱氨酸的生物合成，对细菌的自我防御机制很重要。结核分枝杆菌CysE（Rv2335）已被鉴定为丝氨酸乙酰转移酶，与耻垢分枝杆菌MSMEG_5947是直系同源物。在本研究中，克隆并表达了MSMEG_5947基因，并鉴定其为丝氨酸乙酰转移酶。为了研究耻垢分枝杆菌CysE的功能，通过同源重组构建了MSMEG_5947基因敲除突变株（耻垢分枝杆菌sm-ΔM_5947）。分别使用生长曲线和电子显微镜研究了该菌株的生长和形态特征。耻垢分枝杆菌sm-ΔM_5947的生长比耻垢分枝杆菌mc(2) 155慢。电子显微镜显示，耻垢分枝杆菌CysE蛋白的缺失导致了剧烈的形态变化。因此，丝氨酸乙酰转移酶的缺失会延缓分枝杆菌的生长，但丝氨酸乙酰转移酶的表达对细菌的存活并非必需。
Zotero 插件