Peng Min, Cai Pingping, Ma Hongbo, Meng Hongyan, Xu Yuan, Zhang Xiaoyi, Si Guomin
Department of Traditional Chinese Medicine, Provincial Hospital Affiliated to Shandong University, 324, Jinwu Weiqi Road, Ji'nan, 250021, China.
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):194-204. doi: 10.4314/ajtcam.v11i1.31. eCollection 2014.
Progressive fibrosis accompanies all chronic renal disease, connective tissue growth factor (CTGF,) and platelet-derived growth factor-B, (PDGF-B,) play important roles in extra-cellular matrix abnormal accumulation, while endothelin-1 (ET-1) nitric oxide (NO,) are related to endothelial dysfunction, which mediates the progression of renal fibrosis. Shenqi Detoxification Granule (SDG), a traditional Chinese herbal formula, has been used for treatment of chronic renal failure in clinic for many years.
In order to evaluate the efficacy, and explore the mechanism of SDG to inhibit the progression of renal fibrosis, study was carried out using the adenine-induced Wister rats as the CRF model, and losartan as postive control drug. Levels of serum creatinine [Scr], and blood urea nitrogen (BUN), albumin (ALB), 24hrs, urine protein (24hUP), triacylglycerol (TG), and cholesterol (CHO), together with ET-1, and NO were detected. Pathological changes of renal tissues were observed by HE, staining. In addition, CTGF and PDGF-B expression were analyzed by immuno-histo-chemistry.
The results indicated that SDG can effectively reduce Scr, BUN, 24hUP, TG, and CHO levels, increase ALB levels, inhibit renal tissue damage in CRF rats, and the mechanism maybe reduce PDGF-B, CTGF expression and ET-1/NO.
Shenqi Detoxification Granule is a beneficial treatment for chronic renal failure.
进行性纤维化伴随所有慢性肾脏疾病，结缔组织生长因子（CTGF）和血小板衍生生长因子-B（PDGF-B）在细胞外基质异常积聚中起重要作用，而内皮素-1（ET-1）、一氧化氮（NO）与内皮功能障碍有关，介导肾纤维化的进展。参芪排毒颗粒（SDG）是一种传统中药配方，多年来一直在临床上用于治疗慢性肾衰竭。
为了评估SDG抑制肾纤维化进展的疗效并探索其机制，以腺嘌呤诱导的Wistar大鼠作为慢性肾衰竭模型进行研究，并以氯沙坦作为阳性对照药物。检测血清肌酐[Scr]、血尿素氮（BUN）、白蛋白（ALB）、24小时尿蛋白（24hUP）、甘油三酯（TG）和胆固醇（CHO）水平，以及ET-1和NO水平。通过HE染色观察肾组织的病理变化。此外，通过免疫组织化学分析CTGF和PDGF-B的表达。
结果表明，SDG可有效降低Scr、BUN、24hUP、TG和CHO水平，提高ALB水平，抑制慢性肾衰竭大鼠的肾组织损伤，其机制可能是降低PDGF-B、CTGF表达以及ET-1/NO。
参芪排毒颗粒对慢性肾衰竭是一种有效的治疗方法。
