Misago M, Chiba S, Kikuchi M, Tsukada J, Sato T, Oda S, Eto S
First Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Int J Cell Cloning. 1989 Jan;7(1):39-49. doi: 10.1002/stem.5530070106.
The effects of recombinant human hemopoietic growth factors on early and late human erythroid progenitors (BFU-e and CFU-e) were investigated in serum-free cultures. Recombinant human erythropoietin (rhEpo) induced the formation of not only human CFU-e-derived colonies but also human BFU-e-derived bursts. Recombinant human interleukin 3 (rhIL-3) alone did not induce the formation of human BFU-e-derived bursts and human CFU-e-derived colonies. In the presence of rhEpo, rhIL-3 dose dependently increased the number of bursts stimulated by rhEpo, although rhIL-3 did not have the augmentative effect on human CFU-e growth. On the other hand, rhIL-3 did not stimulate the formation of murine BFU-e-derived bursts, and murine IL-3 did not stimulate the formation of human BFU-e-derived bursts. The results indicated that the burst-promoting activity of IL-3 was species-specific between human and murine cells. Recombinant human GM-CSF (rhGM-CSF) or recombinant human G-CSF (rhG-CSF) failed to induce human burst formation and did not augment the effect of rhEpo on human burst formation. The results of the present study suggest that in vitro, IL-3 can stimulate BFU-e in collaboration with Epo, but GM-CSF and G-CSF do not stimulate BFU-e growth in the presence or absence of Epo.
在无血清培养中研究了重组人造血生长因子对人早期和晚期红系祖细胞（BFU-e和CFU-e）的作用。重组人促红细胞生成素（rhEpo）不仅能诱导人CFU-e来源的集落形成，还能诱导人BFU-e来源的爆式集落形成。单独的重组人白细胞介素3（rhIL-3）不能诱导人BFU-e来源的爆式集落和人CFU-e来源的集落形成。在rhEpo存在的情况下，rhIL-3剂量依赖性地增加了rhEpo刺激的爆式集落数量，尽管rhIL-3对人CFU-e的生长没有增强作用。另一方面，rhIL-3不能刺激鼠BFU-e来源的爆式集落形成，鼠IL-3也不能刺激人BFU-e来源的爆式集落形成。结果表明，IL-3的爆式集落促进活性在人和鼠细胞之间具有种属特异性。重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）或重组人粒细胞集落刺激因子（rhG-CSF）不能诱导人爆式集落形成，也不能增强rhEpo对人爆式集落形成的作用。本研究结果表明，在体外，IL-3可与Epo协同刺激BFU-e，但GM-CSF和G-CSF在有或无Epo的情况下均不能刺激BFU-e生长。
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