Department of Chemical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India.
Department of Biological Science and Bioengineering, Indian Institute of Technology Kanpur, Kanpur 208016, India.
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:46-54. doi: 10.1016/j.msec.2014.01.042. Epub 2014 Jan 31.
In the present study, different proteins, namely, bovine serum albumin (BSA), glucose oxidase (GOx) and the laboratory purified YqeH were immobilized in the phenolic resin precursor-based multi-scale web of activated carbon microfibers (ACFs) and carbon nanofibers (CNFs). These biomolecules are characteristically different from each other, having different structure, number of parent amino acid molecules and isoelectric point. CNF was grown on ACF substrate by chemical vapor deposition, using Ni nanoparticles (Nps) as the catalyst. The ultra-sonication of the CNFs was carried out in acidic medium to remove Ni Nps from the tip of the CNFs to provide additional active sites for adsorption. The prepared material was directly used as an adsorbent for proteins, without requiring any additional treatment. Several analytical techniques were used to characterize the prepared materials, including scanning electron microscopy, Fourier transform infrared spectroscopy, BET surface area, pore-size distribution, and UV-vis spectroscopy. The adsorption capacities of prepared ACFs/CNFs in this study were determined to be approximately 191, 39 and 70 mg/g for BSA, GOx and YqeH, respectively, revealing that the carbon micro-nanofibers forming synthesized multi-scale web are efficient materials for the immobilization of protein molecules.
在本研究中，不同的蛋白质，即牛血清白蛋白（BSA）、葡萄糖氧化酶（GOx）和实验室纯化的 YqeH，被固定在基于酚醛树脂前体的活性炭微纤维（ACF）和碳纳米纤维（CNF）的多尺度网络中。这些生物分子彼此明显不同，具有不同的结构、母体氨基酸分子数量和等电点。通过化学气相沉积，使用镍纳米颗粒（Nps）作为催化剂，在 ACF 基底上生长 CNF。通过超声处理 CNF，在酸性介质中去除 CNF 尖端的 Ni Nps，为吸附提供额外的活性位点。制备的材料未经任何额外处理，直接用作蛋白质的吸附剂。使用多种分析技术对制备的材料进行了表征，包括扫描电子显微镜、傅里叶变换红外光谱、BET 比表面积、孔径分布和紫外-可见光谱。本研究中制备的 ACFs/CNFs 的吸附容量分别约为 191、39 和 70 mg/g，用于 BSA、GOx 和 YqeH，表明形成合成多尺度网络的碳微纳米纤维是固定蛋白质分子的有效材料。
