Bins M, Huiges W, Timmers T, Gelsema E S, Halie M R
Department of Medicine, University of Groningen, The Netherlands.
Blut. 1989 Feb;58(2):79-82. doi: 10.1007/BF00320653.
Neutrophil granulation was quantified after staining with May Grunwald Giemsa or with the pure dyes Azure B and eosin Y. Spinner slides of the buffy coat of 3 normal subjects and 14 persons with different grades of toxic granulation were studied. Morphometric parameters were measured using an image analysis computer (Texture Analysis System, Leitz Wetzlar, FRG). The parameters for granulation varied over a wider range in Azure B (AzB) than in May Grunwald Giemsa (MGG) stained granulocytes. This is in accordance with the visual microscopy observation, that granulation is more pronounced after staining with AzB than MGG. The predominant shade of the nucleus was similar in both stains, whereas considerable and variable differences in the shade of the cytoplasm were found.
用美-格二氏染液或纯染料天青B和伊红Y染色后对中性粒细胞颗粒进行定量分析。研究了3名正常受试者以及14名具有不同程度中毒性颗粒的人员的血沉棕黄层的甩片。使用图像分析计算机（纹理分析系统，德国徕卡·韦茨拉尔公司）测量形态学参数。与美-格二氏（MGG）染色的粒细胞相比，天青B（AzB）染色的粒细胞颗粒参数变化范围更广。这与光学显微镜观察结果一致，即与MGG染色相比，AzB染色后颗粒更明显。两种染色中细胞核的主要色调相似，而细胞质的色调存在显著且可变的差异。
