骨形态发生蛋白4（BMP4）和成纤维细胞生长因子3（FGF3）单倍型会增加排球运动员患肌腱病的风险。

BMP4 and FGF3 haplotypes increase the risk of tendinopathy in volleyball athletes.

作者信息

Salles José Inácio, Amaral Marcus Vinícius, Aguiar Diego Pinheiro, Lira Daisy Anne, Quinelato Valquiria, Bonato Letícia Ladeira, Duarte Maria Eugenia Leite, Vieira Alexandre Rezende, Casado Priscila Ladeira

机构信息

Research Division, National Institute of Traumatology and Orthopaedics, Rio de Janeiro, Brazil; National Teams Department, Brazilian Volleyball Federation, Rio de Janeiro, Brazil.

Research Division, National Institute of Traumatology and Orthopaedics, Rio de Janeiro, Brazil; Center of Shoulder and Elbow Surgery, Department of Orthopaedic Surgery, National Institute of Traumatology and Orthopaedics, Rio de Janeiro, Brazil.

出版信息

J Sci Med Sport. 2015 Mar;18(2):150-5. doi: 10.1016/j.jsams.2014.02.011. Epub 2014 Mar 1.

DOI:10.1016/j.jsams.2014.02.011

PMID:24661680

Abstract

OBJECTIVES

To investigate whether genetic variants can be correlated with tendinopathy in elite male volleyball athletes.

DESIGN

Case-control study.

METHODS

Fifteen single nucleotide polymorphisms within BMP4, FGF3, FGF10, FGFR1 genes were investigated in 138 elite volleyball athletes, aged between 18 and 35 years, who undergo 4-5h of training per day: 52 with tendinopathy and 86 with no history of pain suggestive of tendinopathy in patellar, Achilles, shoulder, and hip abductors tendons. The clinical diagnostic criterion was progressive pain during training, confirmed by magnetic resonance image. Genomic DNA was obtained from saliva samples. Genetic markers were genotyped using TaqMan real-time PCR. Chi-square test compared genotypes and haplotype differences between groups. Multivariate logistic regression analyzed the significance of covariates and incidence of tendinopathy.

RESULTS

Statistical analysis revealed participant age (p=0.005) and years of practice (p=0.004) were risk factors for tendinopathy. A significant association between BMP4 rs2761884 (p=0.03) and tendinopathy was observed. Athletes with a polymorphic genotype have 2.4 times more susceptibility to tendinopathy (OR=2.39; 95%CI=1.10-5.19). Also, association between disease and haplotype TTGGA in BMP4 (p=0.01) was observed. The FGF3 TGGTA haplotype showed a tendency of association with tendinopathy (p=0.05), and so did FGF10 rs900379. FGFR1 showed no association with disease.

CONCLUSIONS

These findings indicate that haplotypes in BMP4 and FGF3 genes may contribute to the tendon disease process in elite volleyball athletes.

摘要

目的

研究基因变异是否与精英男性排球运动员的肌腱病相关。

设计

病例对照研究。

方法

对138名年龄在18至35岁之间、每天训练4 - 5小时的精英排球运动员，研究BMP4、FGF3、FGF10、FGFR1基因中的15个单核苷酸多态性：52例患有肌腱病，86例在髌腱、跟腱、肩腱和髋外展肌腱处无提示肌腱病的疼痛病史。临床诊断标准为训练期间进行性疼痛，并经磁共振成像证实。从唾液样本中获取基因组DNA。使用TaqMan实时聚合酶链反应对基因标记进行基因分型。卡方检验比较组间基因型和单倍型差异。多因素逻辑回归分析协变量的显著性和肌腱病的发病率。

结果

统计分析显示参与者年龄（p = 0.005）和训练年限（p = 0.004）是肌腱病的危险因素。观察到BMP4 rs2761884与肌腱病之间存在显著关联（p = 0.03）。具有多态基因型的运动员患肌腱病的易感性高2.4倍（OR = 2.39；95%CI = 1.10 - 5.19）。此外，观察到疾病与BMP4中的单倍型TTGGA之间存在关联（p = 0.01）。FGF3 TGGTA单倍型显示出与肌腱病相关的趋势（p = 0.05），FGF10 rs900379也是如此。FGFR1与疾病无关联。