间质干细胞衍生的微泡增强大鼠坐骨神经再生：周围神经细胞治疗的新方法。
The mesenchymal stem cell-derived microvesicles enhance sciatic nerve regeneration in rat: a novel approach in peripheral nerve cell therapy.
机构信息
From the Departments of Clinical Sciences (A.R., S.A.), and Pathobiology (A.A.F.), Faculty of Veterinary Medicine, and Department of Cellular and Molecular Biotechnology (N.D.), Institute of Biotechnology, Urmia University; and Neurophysiology Research Center (B.H.), Urmia University of Medical Sciences, Urmia, Iran; and Department of Veterinary Pathology (K.A.), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
出版信息
J Trauma Acute Care Surg. 2014 Apr;76(4):991-7. doi: 10.1097/TA.0000000000000186.
BACKGROUND
The accomplishment for desired functional peripheral nerve regeneration is still challenging despite various materials and methods. The effects of local application of omental adipose mesenchymal stromal cell-derived microvesicles (MVs) on peripheral nerve regeneration were studied using a rat sciatic nerve transection model.
METHODS
A 10-mm gap of sciatic nerve was bridged with a chitosan conduit. The rats were divided into five experimental groups randomly as follows: cultured undifferentiated omental adipose-derived stromal cells, rest mesenchymal stem cell-derived MVs (c-MVs), anti-inflammatory mesenchymal stem cell-derived MVs (anti-MVs), proinflammatory mesenchymal stem cell-derived MVs (pro-MVs), and negative control (Chit).
RESULTS
The functional assessment of nerve regeneration (walking track analyses), electrophysiologic measurements, muscle mass measurements, as well as histomorphometrical and immunohistochemical indices showed drastic improvement in nerve regeneration in c-MVs and anti-MVs animals compared with pro-MVs animals (p < 0.05).
CONCLUSION
The anti-inflammatory stem cell-derived MVs can be used as an alternative for the improvement of rat sciatic nerve regeneration.
背景
尽管有各种材料和方法，但实现理想的周围神经功能再生仍然具有挑战性。本研究使用大鼠坐骨神经横断模型，研究了网膜脂肪间充质基质细胞衍生的微泡（MVs）局部应用对周围神经再生的影响。
方法
用壳聚糖导管桥接 10mm 坐骨神经缺损。将大鼠随机分为五组：未分化的网膜脂肪间充质基质细胞、静止的间充质干细胞衍生的 MVs（c-MVs）、抗炎性的间充质干细胞衍生的 MVs（anti-MVs）、促炎性的间充质干细胞衍生的 MVs（pro-MVs）和阴性对照（Chit）。
结果
神经再生的功能评估（步态分析）、电生理测量、肌肉质量测量以及组织形态计量学和免疫组织化学指标均表明，c-MVs 和 anti-MVs 组动物的神经再生明显优于 pro-MVs 组动物（p < 0.05）。
结论
抗炎性的间充质干细胞衍生的 MVs 可作为改善大鼠坐骨神经再生的替代方法。
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