Department of histology and embryology, Institute of neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
Proteomics. 2014 Jun;14(11):1322-7. doi: 10.1002/pmic.201300532. Epub 2014 Apr 19.
A new fluorescent prestaining method for gel-separated glycoproteins in 1D and 2D SDS-PAGE was developed by using dansylhydrazine in this study. The prestained gels could be easily imaged after electrophoresis without any time-consuming steps needed for poststains. As low as 4-8 ng glycoproteins (transferrin, α1-acid glycoprotein) could be selectively detected, which is comparable to that of Pro-Q Emerald 488, one of the most commonly used glycoprotein stain. In addition, a subsequent study of deglycosylation, glycoprotein affinity isolation, and LC-MS/MS analysis was performed to confirm the specificity of the newly developed method.
本研究开发了一种新的荧光预染方法，用于 1D 和 2D SDS-PAGE 中分离的糖蛋白。该预染方法使用丹磺酰肼（dansylhydrazine），电泳后无需进行任何耗时的后染步骤，即可轻松成像。该方法能够选择性检测低至 4-8ng 的糖蛋白（转铁蛋白、α1-酸性糖蛋白），与最常用的糖蛋白染色剂之一 Pro-Q Emerald 488 相当。此外，还进行了随后的糖基化酶解、糖蛋白亲和分离和 LC-MS/MS 分析研究，以确认新开发方法的特异性。
Zotero 插件