患有胃食管反流病且声带黏膜受到机械损伤的实验大鼠中的喉肉芽肿
Laryngeal granuloma in experimental rats with gastroesophageal reflux disease and mechanically injured vocal cord mucosa.
作者信息
Shimazu Rintaro, Kuratomi Yuichiro, Aoki Shigehisa, Inokuchi Akira
机构信息
Department of Otolaryngology, Head & Neck Surgery, Faculty of Medicine, Saga University, Saga, Japan.
出版信息
Ann Otol Rhinol Laryngol. 2014 Apr;123(4):247-51. doi: 10.1177/0003489414525018.
OBJECTIVE
This study was undertaken to elucidate the mechanisms underlying laryngeal granuloma formation in a rat model of gastroesophageal reflux disease (GERD) with mechanically injured vocal cord mucosa.
METHODS
The rat model of GERD was surgically created by tying the pyloric sphincter and ligating the transitional region between the forestomach and the glandular portion (limiting ridge). The control rats received only a midline incision. In all the animals, a plastic bar was inserted into the trachea, and moved vertically thrice in 3 seconds to cause mechanical injury of the vocal cord mucosa. The rats were sacrificed 2 weeks postsurgically, and their pharynx and larynx were observed histologically.
RESULTS
Granulomas were observed in the vocal cord mucosa of the GERD group (3 of 5 animals); they presented a similar pathological structure to that of human laryngeal granulomas. In contrast, only abrasions and blisters were observed on the vocal cord mucosa in the control group (all 5 animals).
CONCLUSIONS
The development of laryngeal granuloma may involve both mechanical injury and gastric acid reflux.
目的
本研究旨在阐明在声带黏膜受到机械损伤的胃食管反流病（GERD）大鼠模型中喉肉芽肿形成的潜在机制。
方法
通过结扎幽门括约肌和胃前壁与腺部之间的过渡区域（界限嵴），手术创建GERD大鼠模型。对照大鼠仅接受中线切口。在所有动物中，将一根塑料棒插入气管，并在3秒内垂直移动三次，以造成声带黏膜的机械损伤。术后2周处死大鼠，对其咽喉进行组织学观察。
结果
GERD组的5只动物中有3只在声带黏膜中观察到肉芽肿；它们呈现出与人类喉肉芽肿相似的病理结构。相比之下，对照组的所有5只动物的声带黏膜仅观察到擦伤和水泡。
结论
喉肉芽肿的发生可能涉及机械损伤和胃酸反流。
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