Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, China.
College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea.
Neurosci Lett. 2014 May 1;567:19-23. doi: 10.1016/j.neulet.2014.03.018. Epub 2014 Mar 24.
The role of neuropeptide Y (NPY) in the central nucleus of amygdala (CeA) in the preventive effects of acupuncture against ethanol withdrawal-induced anxiety was investigated. Rats were treated with 3g/kg/day of ethanol for 28 days, followed by 3 days of withdrawal. Bilateral acupuncture treatment at HT7 (Shen-Men), PC6 (Nei-Guan) or a non-acupoint was respectively added to the rats during the withdrawal once a day for three days. Enzyme-linked immunosorbent assays and real-time polymerase chain reaction analyses showed there was a significant decrease in NPY protein and mRNA expression in the CeA during ethanol withdrawal, which was reversed by acupuncture at HT7 but neither at PC6 nor at a non-acupoint. Acupuncture at HT7 also greatly inhibited the decrease in cAMP response element-binding protein (CREB) phosphorylation in the CeA. In elevated plus maze tests, a selective NPY Y1 receptor antagonist BIBP 3226 into the CeA before the acupuncture abolished almost completely the anxiolytic effect of acupuncture at HT7. These results suggest that acupuncture at HT7 rescues the depletion of amygdaloid NPY and reverses the decrease in CREB phosphorylation to produce anxiolytic effects during ethanol withdrawal.
研究了神经肽 Y（NPY）在杏仁中央核（CeA）中在针刺预防乙醇戒断引起的焦虑中的作用。大鼠接受 3g/kg/天的乙醇治疗 28 天，然后进行 3 天的戒断。在戒断期间，每天一次在 HT7（神门）、PC6（内关）或非穴位处对大鼠进行双侧针刺治疗，持续三天。酶联免疫吸附测定和实时聚合酶链反应分析显示，乙醇戒断期间 CeA 中的 NPY 蛋白和 mRNA 表达显著下降，针刺 HT7 可逆转这种下降，但针刺 PC6 或非穴位则不能。针刺 HT7 还极大地抑制了 CeA 中 cAMP 反应元件结合蛋白（CREB）磷酸化的减少。在高架十字迷宫测试中，在针刺前将 NPY Y1 受体拮抗剂 BIBP 3226 注入 CeA，几乎完全消除了针刺 HT7 的抗焦虑作用。这些结果表明，针刺 HT7 可挽救杏仁核 NPY 的耗竭，并逆转 CREB 磷酸化的减少，从而在乙醇戒断期间产生抗焦虑作用。
