Montgomery Eric, Gao Chen, de Luca Julie, Bower Jessie, Attwood Kristropher, Ylagan Lourdes
Department of Pathology and Laboratory Medicine, Vassar Brothers Medical Center, Poughkeepsie, New York.
Diagn Cytopathol. 2014 Dec;42(12):1024-33. doi: 10.1002/dc.23155. Epub 2014 Mar 27.
The Cellient(®) cell block system has become available as an alternative, partially automated method to create cell blocks in cytology. We sought to show a validation method for immunohistochemical (IHC) staining on the Cellient cell block system (CCB) in comparison with the formalin fixed paraffin embedded traditional cell block (TCB). Immunohistochemical staining was performed using 31 antibodies on 38 patient samples for a total of 326 slides. Split samples were processed using both methods by following the Cellient(®) manufacturer's recommendations for the Cellient cell block (CCB) and the Histogel method for preparing the traditional cell block (TCB). Interpretation was performed by three pathologists and two cytotechnologists. Immunohistochemical stains were scored as: 0/1+ (negative) and 2/3+ (positive). Inter-rater agreement for each antibody was evaluated for CCB and TCB, as well as the intra-rater agreement between TCB and CCB between observers. Interobserver staining concordance for the TCB was obtained with statistical significance (P < 0.05) in 24 of 31 antibodies. Interobserver staining concordance for the CCB was obtained with statistical significance in 27 of 31 antibodies. Intra-observer staining concordance between TCB and CCB was obtained with statistical significance in 24 of 31 antibodies tested. In conclusions, immunohistochemical stains on cytologic specimens processed by the Cellient system are reliable and concordant with stains performed on the same split samples processed via a formalin fixed-paraffin embedded (FFPE) block. The Cellient system is a welcome adjunct to cytology work-flow by producing cell block material of sufficient quality to allow the use of routine IHC.
Cellient（®）细胞块系统已成为一种可替代的、部分自动化的方法，用于在细胞学中制作细胞块。我们试图展示一种在Cellient细胞块系统（CCB）上进行免疫组织化学（IHC）染色的验证方法，并与福尔马林固定石蜡包埋的传统细胞块（TCB）进行比较。使用31种抗体对38例患者样本进行免疫组织化学染色，共制作326张玻片。按照Cellient（®）制造商对Cellient细胞块（CCB）的建议以及Histogel方法制备传统细胞块（TCB），对分割样本采用两种方法进行处理。由三名病理学家和两名细胞技术人员进行解读。免疫组织化学染色评分如下：0/1+（阴性）和2/3+（阳性）。评估了CCB和TCB中每种抗体的评分者间一致性，以及观察者之间TCB和CCB的评分者内一致性。31种抗体中有24种，TCB的观察者间染色一致性具有统计学意义（P < 0.05）。31种抗体中有27种，CCB的观察者间染色一致性具有统计学意义。在31种测试抗体中，有24种TCB和CCB之间的观察者内染色一致性具有统计学意义。总之，通过Cellient系统处理的细胞学标本上的免疫组织化学染色是可靠的，并且与通过福尔马林固定石蜡包埋（FFPE）块处理的相同分割样本上的染色一致。Cellient系统通过生产质量足够高的细胞块材料，使常规IHC得以应用，是细胞学工作流程中一项受欢迎的辅助手段。
