Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada; Department of Biology, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada.
Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada.
J Microbiol Methods. 2014 Jun;101:28-32. doi: 10.1016/j.mimet.2014.03.007. Epub 2014 Mar 26.
An anaerobic colorimetric assay for quantifying microbial demethylation activity was adapted for aerobic use in studying lignin and lignin-derived compounds. Standard curves of 0-500μM pyrocatechol with and without 0.3% lignin demonstrated the use in either case. This method detects demethylation products up to 500μM without using additional dilutions.
本研究将一种用于定量检测微生物去甲基化活性的厌氧比色法进行改良，使之可用于研究木质素和木质素衍生化合物的好氧条件下的相关研究。在有和没有 0.3%木质素的情况下，0-500μM 邻苯二酚的标准曲线均证明了这两种情况下的使用。该方法无需额外稀释，即可检测高达 500μM 的去甲基化产物。
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