一种含牛磺酸席夫碱和咪唑的混合配体铜（II）配合物的DNA结合、细胞毒性及诱导凋亡活性
DNA binding, cytotoxicity and apoptosis induction activity of a mixed-ligand copper(II) complex with taurine Schiff base and imidazole.
作者信息
Li Mei, Kong Lin Lin, Gou Yi, Yang Feng, Liang Hong
机构信息
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, China; State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guangxi Normal University, Guilin, Guangxi, China.
State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guangxi Normal University, Guilin, Guangxi, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:686-93. doi: 10.1016/j.saa.2014.02.197. Epub 2014 Mar 14.
A novel binuclear copper(II) complex (complex 1) with taurine Schiff base and imidazole has been synthesized and structurally characterized by single crystal X-ray diffraction, elemental analysis, ESI-MS spectrometry, UV-vis and IR spectroscopy. Single-crystal analysis revealed that 1 displays the sulfonate-bridged dinuclear copper(II) centers. Both copper atoms are five-coordinated and exhibit slightly distorted square pyramidal geometries. Each of copper atom is surrounded by three oxygen atoms and one nitrogen atom from different taurine Schiff base ligands, and one nitrogen atom from one imidazole ligand. The interaction between 1 and calf thymus DNA (CT-DNA) was investigated by UV-vis, fluorescence, circular dichroism (CD) spectra and agarose gel electrophoresis. The experimental results indicated that 1 could bind to CT-DNA via an intercalative mode and show efficient cleavage activity. In addition, 1 showed an antitumor effect on cell cycle and apoptosis. Flow cytometric analysis revealed that MGC-803 cells were arrested in the S phase after treatment with 1. Fluorescence microscopic observation indicated that 1 could induce apoptosis of MGC-803 cells.
合成了一种新型的含牛磺酸席夫碱和咪唑的双核铜（II）配合物（配合物1），并通过单晶X射线衍射、元素分析、电喷雾电离质谱、紫外可见光谱和红外光谱对其进行了结构表征。单晶分析表明，1呈现出磺酸根桥联的双核铜（II）中心。两个铜原子均为五配位，呈现出略微扭曲的四方锥几何构型。每个铜原子被来自不同牛磺酸席夫碱配体的三个氧原子和一个氮原子以及来自一个咪唑配体的一个氮原子所包围。通过紫外可见光谱、荧光光谱、圆二色光谱（CD）和琼脂糖凝胶电泳研究了1与小牛胸腺DNA（CT-DNA）之间的相互作用。实验结果表明，1能够通过插入模式与CT-DNA结合并表现出高效的切割活性。此外，1对细胞周期和凋亡显示出抗肿瘤作用。流式细胞术分析表明，用1处理后，MGC-803细胞停滞在S期。荧光显微镜观察表明，1能够诱导MGC-803细胞凋亡。
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