Mehri R, Mavriplis C, Fenech M
J Biomech Eng. 2014 Jun;136(6):064501. doi: 10.1115/1.4027351.
The purpose of this paper is to design a microfluidic apparatus capable of providing controlled flow conditions suitable for red blood cell (RBC) aggregation analysis. The linear velocity engendered from the controlled flow provides constant shear rates used to qualitatively analyze RBC aggregates. The design of the apparatus is based on numerical and experimental work. The numerical work consists of 3D numerical simulations performed using a research computational fluid dynamics (CFD) solver, Nek5000, while the experiments are conducted using a microparticle image velocimetry system. A Newtonian model is tested numerically and experimentally, then blood is tested experimentally under several conditions (hematocrit, shear rate, and fluid suspension) to be compared to the simulation results. We find that using a velocity ratio of 4 between the two Newtonian fluids, the layer corresponding to blood expands to fill 35% of the channel thickness where the constant shear rate is achieved. For blood experiments, the velocity profile in the blood layer is approximately linear, resulting in the desired controlled conditions for the study of RBC aggregation under several flow scenarios.
本文的目的是设计一种微流控装置，该装置能够提供适合红细胞（RBC）聚集分析的可控流动条件。由可控流动产生的线性速度提供了用于定性分析RBC聚集体的恒定剪切速率。该装置的设计基于数值和实验工作。数值工作包括使用研究计算流体动力学（CFD）求解器Nek5000进行的三维数值模拟，而实验则使用微粒图像测速系统进行。对牛顿模型进行了数值和实验测试，然后在几种条件（血细胞比容、剪切速率和流体悬浮液）下对血液进行了实验测试，以便与模拟结果进行比较。我们发现，使用两种牛顿流体之间4的速度比，对应于血液的层会扩展以填充通道厚度的35%，在此处可实现恒定剪切速率。对于血液实验，血液层中的速度分布大致呈线性，从而为在几种流动场景下研究RBC聚集提供了所需的可控条件。
