Target regulation of the serotonin and substance P innervation of the sexually dimorphic cremaster nucleus.

The effects of perinatal castrations on the serotonin (5-HT) and substance P (SP) innervation patterns of the adult male cremaster nucleus (CN) were examined in Sprague-Dawley rats. These investigations reveal that day-of-birth bilateral castration precludes cremaster muscle formation, significantly reduces CN motoneuron number, and dramatically reduces the 5-HT and SP innervation of the adult male CN. Day-of-birth castration reduces the adult CN SP innervation pattern to a greater degree than the 5-HT pattern and this may be attributed to a substantial loss of primary afferent information to the dorsal horn of the spinal cord. Furthermore, day-of-birth unilateral castration causes an ipsilateral reduction in the adult 5-HT and SP immunostaining patterns. This may be correlated with the failure of the ipsilateral cremaster muscle to form with a subsequent ipsilateral reduction of CN motoneuron number. On the contralateral side the intact testis is accompanied by normal cremaster muscle gross morphology, CN motoneuron number, and CN immunostaining. Together these observations suggest that the sexually dimorphic 5-HT and SP innervation of the male CN is predominantly under target control vs androgen control. Castration during adulthood does not alter the 5-HT and SP innervation patterns of CN; thus, a time-dependent period, probably initiated by cremaster muscle formation, underlies these target organ regulatory effects.