Davis B H, Bigelow N C
Department of Pathology, Dartmouth-Hitchcock Medical Center, Hanover, NH 03756.
Arch Pathol Lab Med. 1989 Jun;113(6):684-9.
Flow cytometric reticulocyte quantification with thiazole orange has been reported to be of potential utility in a clinical hematology laboratory. We have instituted this technique into routine clinical testing for 18 months and we describe this experience. Flow cytometric analysis provided not only reproducible, cost-effective reticulocyte quantification, but a quantitative reticulocyte maturity index proportional to the amount of RNA in the reticulocytes. The reticulocyte maturity index measurement represents an independent parameter of erythropoiesis, which provided clinically valuable information regarding bone marrow engraftment in patients following autologous bone marrow transplantation. The findings of this study demonstrate the clinical utility of thiazole orange reticulocyte analysis and indicate the diagnostic importance of the reticulocyte maturity index measurement in the evaluation of erythropoietic activity.
据报道，使用噻唑橙进行流式细胞术网织红细胞定量在临床血液学实验室具有潜在用途。我们已将该技术应用于常规临床检测18个月，并在此描述我们的经验。流式细胞术分析不仅提供了可重复、具有成本效益的网织红细胞定量，还提供了与网织红细胞中RNA含量成比例的定量网织红细胞成熟指数。网织红细胞成熟指数测量代表了红细胞生成的一个独立参数，它为自体骨髓移植后患者的骨髓植入提供了具有临床价值的信息。本研究结果证明了噻唑橙网织红细胞分析的临床实用性，并表明网织红细胞成熟指数测量在评估红细胞生成活性中的诊断重要性。
