智利患者胃癌中P2RY2、CD248和EphB1表达增加。
Increased expression of P2RY2, CD248 and EphB1 in gastric cancers from Chilean patients.
作者信息
Aquea Gisela, Bresky Gustavo, Lancellotti Domingo, Madariaga Juan Andres, Zaffiri Vittorio, Urzua Ulises, Haberle Sergio, Bernal Giuliano
机构信息
Departamento de Ciencias Biomedicas, Facultad de Medicina, Universidad Catolica del Norte, Coquimbo, Chile E-mail :
出版信息
Asian Pac J Cancer Prev. 2014;15(5):1931-6. doi: 10.7314/apjcp.2014.15.5.1931.
BACKGROUND
Gastric cancer (GC) ranks as one of the major causes of mortality due to cancer worldwide. In Chile, it is currently the leading cause of cancer death. Identification of novel molecular markers that may help to improve disease diagnosis at early stages is imperative.
MATERIALS AND METHODS
Using whole-genome DNA microarrays we determined differential mRNA levels in fresh human GC samples compared to adjacent healthy mucosa from the same patients. Genes significantly overexpressed in GC were validated by RT-PCR in a group of 14 GC cases.
RESULTS
The genes CD248, NSD1, RAB17, ABCG8, Ephb1 and P2RY2 were detected as the top overexpressed in GC biopsies. P2RY2, Ephb1 and CD248 showed the best sensitivity for GC detection with values of 92.9%, 85.7% and 64.3% (p<0.05), respectively. Specificity was 85.7%, 71.4% and 71.4% (p<0.05), for each respectively.
背景
胃癌（GC）是全球癌症死亡的主要原因之一。在智利，它目前是癌症死亡的首要原因。识别可能有助于改善疾病早期诊断的新型分子标志物势在必行。
材料与方法
我们使用全基因组DNA微阵列，测定了新鲜人类胃癌样本与同一患者相邻健康黏膜相比的差异mRNA水平。在一组14例胃癌病例中，通过逆转录聚合酶链反应（RT-PCR）对在胃癌中显著过表达的基因进行了验证。
结果
基因CD248、NSD1、RAB17、ABCG8、Ephb1和P2RY2被检测为在胃癌活检中过表达最显著的基因。P2RY2、Ephb1和CD248对胃癌检测的敏感性最佳，分别为92.9%、85.7%和64.3%（p<0.05）。特异性分别为85.7%、71.4%和71.4%（p<0.05）。
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