[人表皮生长因子受体2基因在晚期胃癌中的扩增与过表达]

[HER2 gene amplification and overexpression in advanced gastric cancer].

作者信息

Roa Iván, Slater Jeannie, Carvajal Daniel, Schalper Kurt, de Toro Gonzalo, Ares Raúl, Game Anakaren, León Jorge, de Aretxabala Xabier

出版信息

Rev Med Chil. 2013 Nov;141(11):1411-9. doi: 10.4067/S0034-98872013001100007.

DOI:10.4067/S0034-98872013001100007

PMID:24718467

Abstract

BACKGROUND

Overexpression/amplification of the HER2 gene in advanced gastric cancer is a predictor of response to adjuvant therapy with monoclonal antibodies.

AIM

To determine the frequency of HER2 gene overexpression and amplification in advanced gastric cancer.

MATERIAL AND METHODS

One hundred nine advanced gastric cancer biopsy specimens, from 76 men and 33 women aged 67 ± 14 and 62 ± 12 years respectively, were selected. Three histological patterns (diffuse, intestinal and mixed) were recognized. Automated immunohistochemistry was performed with monoclonal c-erbB-2 (NCL-356) Novocastra. Fluorescent in situ hybridization (FISH) for HER2 was performed in positive cases.

RESULTS

In 39% of cases, immunohistochemical staining was negative. It was 1+, 2+ and 3+ positive in 15, 36 and 11% of cases, respectively. It was positive in 16% and 3% of intestinal type and mixed carcinomas, respectively. It was negative in all diffuse carcinomas. FISH was performed in 39 (2 +) cases and in 11 (3 +) cases. The gene amplification was positive in two (2 +) and 11 (3 +) cases (11.9%). The overall concordance between immunohistochemical staining and in situ hybridization was 85%.

CONCLUSIONS

In advanced gastric cancer, HER2 gene overexpression or amplification was observed in 11% and 12% of cases, respectively.

摘要

背景

HER2基因在晚期胃癌中的过表达/扩增是单克隆抗体辅助治疗反应的预测指标。

目的

确定晚期胃癌中HER2基因过表达和扩增的频率。

材料与方法

选取109例晚期胃癌活检标本，分别来自76名男性和33名女性，年龄分别为67±14岁和62±12岁。识别出三种组织学模式（弥漫型、肠型和混合型）。使用Novocastra的单克隆c-erbB-2（NCL-356）进行自动免疫组织化学检测。对阳性病例进行HER2的荧光原位杂交（FISH）检测。

结果

39%的病例免疫组织化学染色为阴性。15%、36%和11%的病例分别为1+、2+和3+阳性。肠型和混合型癌的阳性率分别为16%和3%。所有弥漫型癌均为阴性。对39例（2+）病例和11例（3+）病例进行了FISH检测。2例（2+）和11例（3+）病例（11.9%）基因扩增呈阳性。免疫组织化学染色与原位杂交的总体一致性为85%。