用于细胞尺度核磁共振波谱的原子力显微镜耦合微线圈
Atomic force microscopy-coupled microcoils for cellular-scale nuclear magnetic resonance spectroscopy.
作者信息
Mousoulis Charilaos, Maleki Teimour, Ziaie Babak, Neu Corey P
机构信息
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Department of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Appl Phys Lett. 2013 Apr 8;102(14):143702. doi: 10.1063/1.4801318. Epub 2013 Apr 11.
We present the coupling of atomic force microscopy (AFM) and nuclear magnetic resonance (NMR) technologies to enable topographical, mechanical, and chemical profiling of biological samples. Here, we fabricate and perform proof-of-concept testing of radiofrequency planar microcoils on commercial AFM cantilevers. The sensitive region of the coil was estimated to cover an approximate volume of 19.4 × 10 m (19.4 pl). Functionality of the spectroscopic module of the prototype device is illustrated through the detection of Η resonance in deionized water. The acquired spectra depict combined NMR capability with AFM that may ultimately enable biophysical and biochemical studies at the single cell level.
我们展示了原子力显微镜（AFM）与核磁共振（NMR）技术的耦合，以实现对生物样品的形貌、力学和化学分析。在此，我们在商用AFM悬臂上制造并进行了射频平面微线圈的概念验证测试。估计该线圈的敏感区域覆盖的体积约为19.4×10⁻¹² m³（19.4皮升）。通过检测去离子水中的氢共振，说明了原型设备光谱模块的功能。所获得的光谱描绘了NMR与AFM相结合的能力，这最终可能实现单细胞水平的生物物理和生化研究。
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