α-异头寡核苷酸对莫洛尼鼠白血病病毒逆转录酶的抑制作用。

Inhibition of Moloney murine leukemia virus reverse transcriptase by alpha-anomeric oligonucleotides.

作者信息

Lavignon M, Bertrand J R, Rayner B, Imbach J L, Malvy C, Paoletti C

机构信息

UA 147 CNRS, U 140 INSERM, Institut Gustave Roussy, Villejuif, France.

出版信息

Biochem Biophys Res Commun. 1989 Jun 30;161(3):1184-90. doi: 10.1016/0006-291x(89)91367-3.

DOI:10.1016/0006-291x(89)91367-3

PMID:2472790

Abstract

After parallel hybridization to complementary template RNA, alpha-anomeric oligonucleotides are not primers for Moloney murine leukemia virus reverse transcriptase. As can be expected they are competitors of classical primer oligonucleotides (beta-anomeric). They therefore inhibit the RNA dependent DNA polymerase activity of Moloney murine leukemia virus reverse transcriptase with either homopolymeric or heteropolymeric substrates. Non complementary alpha-oligonucleotides display no inhibitory activity. alpha-Oligonucleotides are therefore potential candidates for inhibition of retroviral reverse transcriptases by interference with the primer binding sites.

摘要

与互补模板RNA进行平行杂交后，α-异头寡核苷酸不是莫洛尼鼠白血病病毒逆转录酶的引物。可以预料，它们是经典引物寡核苷酸（β-异头）的竞争者。因此，它们在同聚物或杂聚物底物存在时，均能抑制莫洛尼鼠白血病病毒逆转录酶的RNA依赖性DNA聚合酶活性。非互补的α-寡核苷酸不显示抑制活性。因此，α-寡核苷酸有可能通过干扰引物结合位点来抑制逆转录病毒逆转录酶。