Yuan Wen-Su, Xu Li-Juan, Liu Meng-Dan, Wang Yue-Bing, Li Dong
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2014 Jan;30(1):14-7.
To test the different contrctile responses of extracellular nucleotides, such as ATP, UTP and nucleotide uridine adenosine tetraphosphate (Up4A) in gastric longitudinal muscle (LM) and circular muscle (CM). Examined the effect of P2X and P2Y receptor antagonists (in this study, we used IP5I and suramin) and cyclooxygenase inhibitor (indomethacin) on Up4A induced contractile responses in LM and CM.
The rats were sacrificed and the stomachs were opened to gain LM and CM. Using organ bath system to assess contrctile responses of smooth muscle.
Up4A could induce contractile responses in both CM and LM, which were similar with ATP and UTP. IP5 did not attenuate Up4A could induce contractions in both LM and CM, but suramin and indomethacin significantly inhibited Up4A contraction in CM, but not in LM.
Our results suggest that extracellular nucleosides and their inhibitors induce different responses between LM and CM.
测试细胞外核苷酸，如三磷酸腺苷（ATP）、尿苷三磷酸（UTP）和四磷酸尿苷腺苷（Up4A）在胃纵行肌（LM）和环行肌（CM）中的不同收缩反应。研究P2X和P2Y受体拮抗剂（在本研究中，我们使用了IP5I和苏拉明）以及环氧合酶抑制剂（吲哚美辛）对Up4A诱导的LM和CM收缩反应的影响。
处死大鼠，打开胃获取LM和CM。使用器官浴系统评估平滑肌的收缩反应。
Up4A可诱导CM和LM产生收缩反应，这与ATP和UTP相似。IP5不能减弱Up4A诱导的LM和CM收缩，但苏拉明和吲哚美辛可显著抑制CM中Up4A的收缩，而对LM无此作用。
我们的结果表明，细胞外核苷及其抑制剂在LM和CM之间诱导不同反应。
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