Girotra Neha, Vandana K L
Department of Periodontics, College of Dental Sciences, Davangere, Karnataka, India.
J Indian Soc Periodontol. 2014 Jan;18(1):26-31. doi: 10.4103/0972-124X.128195.
Fluorosis causes mineralization changes in the tooth and can lead to morphologic alterations of fibroblasts. To understand the effect of fluorosis on periodontal healing, the initial step during healing, such as fibroblast attachment to the root surface, needs to be evaluated. Hence, the objective of the present study was to study the attachment of fluorosed fibroblasts on the fluorosed and nonfluorosed root fragments.
A total of 56 fluorosed and nonfluorosed, periodontally healthy and diseased tooth roots were obtained and allotted to eight groups: Fluorosed and nonfluorosed healthy controls (FH and NFH, respectively), fluorosed and nonfluorosed diseased controls (FD and NFD, respectively), fluorosed and nonfluorosed roots treated with scaling and root planing (FD + S and NFD + S, respectively), and similar groups treated with scaling and root planing and 24% ethylenediaminetetraacetic acid (EDTA) gel application for 2 min (FD + SE and NFD + SE, respectively). After the respective treatment, the root fragments were incubated in the human periodontal ligament fibroblast cells obtained and cultured from freshly extracted healthy human fluorosed premolar tooth root.
In the nonfluorosed roots category, greater attachment was found in the untreated nonfluorosed diseased (P = 0.036) and SRP-treated nonfluorosed diseased groups (P = 0.008) as compared to the nonfluorosed healthy group. While in the fluorosed roots category, no significant difference was observed in FL-FA (P > 0.05) within the group. However, no attachment was observed in EDTA-treated fluorosed root fragments. When fluorosed groups were compared to nonfluorosed groups, no significant changes were noted between the groups.
SRP proves to be a standard requirement for fibroblast attachment to occur both in fluorosed and nonfluorosed roots. Although there was no significant difference in attachment between SRP and SRP + EDTA among fluorosed roots, EDTA does not seem to be a promising agent for root biomodification in fluorosed roots in a given concentration and time of treatment.
氟中毒会导致牙齿矿化改变，并可能导致成纤维细胞形态改变。为了解氟中毒对牙周愈合的影响，需要评估愈合过程中的初始步骤，如成纤维细胞与根面的附着。因此，本研究的目的是研究氟中毒的成纤维细胞在氟中毒和未氟中毒的牙根片段上的附着情况。
共获取56颗氟中毒和未氟中毒、牙周健康和患病的牙根，并分为八组：氟中毒和未氟中毒健康对照组（分别为FH和NFH）、氟中毒和未氟中毒患病对照组（分别为FD和NFD）、经龈下刮治和根面平整治疗的氟中毒和未氟中毒牙根组（分别为FD + S和NFD + S），以及经龈下刮治和根面平整治疗并应用24%乙二胺四乙酸（EDTA）凝胶2分钟的类似组（分别为FD + SE和NFD + SE）。经过相应治疗后，将牙根片段在从新鲜拔除的健康人氟中毒前磨牙牙根中获取并培养的人牙周膜成纤维细胞中孵育。
在未氟中毒牙根类别中，与未氟中毒健康组相比，未治疗的未氟中毒患病组（P = 0.036）和经龈下刮治和根面平整治疗的未氟中毒患病组（P = 0.008）的附着情况更好。而在氟中毒牙根类别中，组内氟中毒组（FL - FA）未观察到显著差异（P > 0.05）。然而，在经EDTA处理的氟中毒牙根片段上未观察到附着情况。当将氟中毒组与未氟中毒组进行比较时，两组之间未观察到显著变化。
龈下刮治和根面平整被证明是氟中毒和未氟中毒牙根上成纤维细胞附着发生的标准要求。尽管在氟中毒牙根中，龈下刮治和根面平整与龈下刮治和根面平整加EDTA之间的附着情况无显著差异，但在给定的治疗浓度和时间下，EDTA似乎不是氟中毒牙根根生物改性的有效药物。
